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- Effects of Temperature Induction Modes on the Plasmid Copy Number in Escherichia coli 温度诱导模式对重组大肠杆菌质粒拷贝数的影响
- Keywords Saccharomyces cerevisiae;expression system;homologous recombination;yeast episomal plasmid;plasmid stability;plasmid copy number;fusion protein; 酿酒酵母;表达系统;同源重组;附加体型载体;质粒稳定性;质粒拷贝数;融合蛋白;
- plasmid copy number 质粒拷贝数
- Methods:We investigated chromosomal DNA copy number changes in 3 ESCC cell lines by comparative genomic hybridization(CGH). 方法:采用比较基因组杂交法(CGH)分析3种ESCC细胞系的染色体DNA拷贝数改变情况。
- DNA sequence copy number changes in benign PTs: Genomic losses prevalently involved chromosome arms 9q34 (2/3) . 良性乳腺叶状肿瘤的染色体DNA拷贝数的变化:共同染色体丢失区为9q34(2/3)。
- Keywords Zymomonas mobilis;Shuttle plasmid;Stability;Copy number; 运动发酵单胞菌;穿梭质粒;稳定性;拷贝数;
- Coincidentally, the psoriasis paper mentioned a recent study that linked SLE to low copy number of complement C4 genes. 巧合的是,银屑病论文提到近期一项研究将SLE与补体蛋白C4基因拷贝数低联系起来。
- He suspects that higher-resolution techniques will reveal that all monozygotic twins have copy number changes. 他猜测解析度更高的技术,将可揭露所有的同卵双胞胎都有基因份数的差异。
- CGH analysis indicated copy number gains of 1p1, 1q2-4,2p1,2q1,5p,7p14,7q21,11q1,15q2,20q and losses of 2p2,2q2,3p,4,9p,14,18,Xq. 比较基因组杂交结果得出染色体 1p1、1q2 - 4、2 p1、2 q1、5 p、7p14、7q2 1、11q1、15 q2、2 0 q扩增 ,其中 5 p表现出高水平的扩增。
- Methods: CGH was used to analyse chromosomal copy number changes in 3 cases of benign PTs, bordline PTs and malignant PTs , respectively. 方法:本课题应用CGH技术分析3例良性叶状肿瘤、3例交界性叶状肿瘤、3例恶性叶状肿瘤的染色体DNA拷贝数的变化情况。
- The Hap Map and Copy Number ariation (CN) Projects deeloped indices of where to find differences in the manuals of different people. 人类基因组单体型图和变异基因拷贝数工程的发展为在何处以及从不同人们的“手册”中找到差异提供了指示物。
- It was found that these genes have low rate of DNA copy number amplification in OPL and HRP than in OSCC, but the amplification is detectable. 发现各基因在口腔癌前病变及口腔癌高危险群中增幅异常之发生率远比在口腔鳞状上皮癌低,但却可以侦测的到。
- High copy number of MET seemed to correlate with shorter TTF in patients with gefitinib-sensitive activating EGFR mutations. 对于吉非替尼敏感性激活EGFR突变患者,MET基因的高拷贝数可能是影像治疗失败时间的不利因素(缩短治疗失败时间)。
- Our study shows, some of these oligonucleotides have a large copy number in genomes while maintain its taxon specificity. 我们的研究表明,有些寡核苷酸重复序列在保持较高复制份数的同时具有很 强的分类特异性。
- Conclusion T4 lysozyme was successfully induced and expressed in Pichia pastoris.There is no relationship between copy number and expression. 加热煮沸对于T4溶菌酶蛋白的抗菌活性无明显影响.;结论T4溶菌酶在毕赤酵母中得以成功诱导与表达;
- DEFB gene products are small antimicrobial proinflammatory peptides, and copy number variation (CNV) could reasonably influence susceptibility to infection and inflammation. DEFB基因产物是小分子抗菌肽,且拷贝数变化(CNV)可显著影响易感性及炎症反应。
- Methods Comparative genomic hybridization(CGH)was used in screening DNA copy number changes along all chromosomes in 23 cases of primary gastric cancer. 方法采用比较基因组杂交分析23例原发性胃癌基因组DNA拷贝数变化情况。
- Conclusion: The method combined DOP-PCR with CGH can realize the cytogenetic analysis of the human genome from low copy number, and the analysis result is accurate and reliable. 结论:本研究所建立的单细胞全基因组扩增联合比较基因组杂交技术适用于单细胞等低拷贝样本的分析,分析结果准确、可靠。
- Objective To prepare DNA probe which can be used to accurately detect chromosome 21 copy number in human interphase nuclei by fluorescence in situ hybridization (FISH). 目的制备能精确检测人类间期细胞核中21号染色体拷贝数的FISH探针。
- Stability and copy number in host bacteria of a new plasmid cloning vector 一种新的质粒克隆载体的稳定性和拷贝数的测定