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- The plasmid expression vector aiming directly at cerb-B1 gene might suppress cerb-B1 gene and EGFR expression in human colon cancer LoVo cells. The new therapeutic modalities could be used in the treatment for human colon cancer. 说明本研究构建的针对cerb-B1基因的质粒表达载体可以显著抑制cerb-B1基因和EGFR蛋白在人结肠癌LoVo细胞的表达,为结肠癌的基因治疗提供了新的思路。
- Lactococcus plasmid expression vector pNZ2103 乳酸菌质粒表达载体pNZ2103
- plasmid expression vector 质粒表达载体
- A doublegene plant expression vector,pC35SC35SB1303 were reconstructed based on PC1303 plasmid by CMO and BADH which were both driven by 35S promoter. 以pC1303质粒为基础,构建了均由35S启动子驱动的CMO基因和BADH基因的植物双基因表达载体pC35SC35SB1303。
- A mammalian expression vector(pSV_2,-EP) was reconstructed by inserting a oligonucleolide fragment into pSV_2dhfr plasmid. 对pSV_2dhfr质粒进行改造,切除dhfr基因,加入一个人工合成的寡聚核苷酸片段,构建成哺乳动物细胞表达载体(pSV_2-EP)。
- ALR gene was also inserted to bicistronic plasmid p! RES2-EGFP to produce ALR, EGFP gene bicistronic eukaryotic expression vector (pIRES-EGFP/ALR). ALR和EGFP基因双顺反子表达载体,pIRES-EGFP/ALR。
- Methods: Eukaryotic expression vector plasmid containing AIRE cDNA was transfected into HeLa cells with FuGENE6 Transfection Reagent. 方法 :用 Fu GENE6转染试剂将含 AIRE c DNA的真核表达载体转染 He La细胞 ,经 G41 8筛选阳性克隆。
- Then the BLY cDNA fragment was subcloned into prokaryotic expression vector pGEX-4T-1, forming the prokaryotic expression plasmid (pG-BLY). 克隆PCR产物,并构建了pGEX-4T-1-BLY原核表达载体。 经BamHI和EcoRI酶切及质粒PCR鉴定,证实本实验构建的新型牛溶菌酶基因已克隆到原核表达载体pGEX-4T-1上,为进一步研究其诱导表达条件及生物学功能奠定了基础。
- PCR amplified green fluorescent protein (GFP) gene was inserted into pGEX-KG expression vector, and resulting plasmid was designated as pKG-GFP. 用PCR扩增出绿色荧光蛋白(GFP)基因,插入到pGEX-KG表达载体中,并将构建出的重组质粒命名为pKG-GFP。
- LCB1 gene was cloned into inducible expression vector pYES2 and transformed into Saccharomyces cerevisiae FY2 The plasmid was induced to express with galactose. 酿酒酵母 (Saccharomycescerevisiae)LCB1 (Longchainbase)基因被克隆到酵母诱导表达载体pYES2中 ,并转入到FY2中 ,用半乳糖诱导表达。
- Characteristics of transfection efficiency and transient expression of plasmid expression vectors coding green fluorescent protein gene transferred into SACC-83 绿荧光蛋白基因质粒表达载体对SACC-83的转染效率及瞬时表达特点
- The choEW was inserted into prokaryotic expression vector pET-His, and the resulting recombinant plasmid pETW was used to transform E. colt BL21(DE3)plysS. 将choEW插入到原核表达载体pET-His中,构建出重组质粒pETW,并转化Escherichia coli BL21(DE3)plysS获得工程菌。
- Methods:(CTP)_4coding gene from pBSMR-(CTP)_4 was cloned into prokaryotic expression vector pET-28a(+) and the expression plasmid pET-28a(+)- (CTP)_4was obtained. 方法:将pBSMR-(CTP)_4中的(CTP)_4基因片断克隆入表达载体pET-28a(+),得到表达质粒pET-28a(+)-(CTP)_4。
- Objective To construct the plasmid expressing BDV p40 gene. 目的构建博尔纳病病毒p40基因重组表达质粒。
- The identified cDNA was cloned into the new type of prokaryotic expression vector pQE-80L, and a synthetized expression plasmid named pQE-80L/DHFR/ABP obtained. The E. 将此基因克隆到原核表达载体pQE-80L,获得融合表达质粒pQE-80L/DHFR/ABP,在1%25IPTG诱导下进行表达。
- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题: 图1.;穿梭表达载体pRL_hEGF的构建。
- And cloning to the expression vector pET-32a(+) fused with Trx-Tag coding sequence or not, to acquire the recombinant plasmid trx-fnb- pET-32a(+), trx-cna- pET-32a(+),fnb- pET-32a(+) and cna- pET-32a(+). 并克隆于原核表达载体pET-32a(+),分别构建了融合基因表达质粒trx-fnb-pET-32a(+),trx-cna-pET-32a(+)和非融合基因表达质粒fnb-pET-32a(+),cna-pET-32a(+);
- Objective To construct the recombinant plasmid of eukaryotic expression vector containing M-CSF gene and transfect it into the NIH3T3 cells to study whether resulting in the expression of M-CSF and EGFP fusion proteins. 目的构建真核表达重组体pEGFP-M-CSF,并鉴定其在小鼠成纤维细胞NIH3T3细胞能否正确表达。
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功构建pPIC9K/Ang-1毕氏酵母表达载体。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。