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- In our studying, we not only used the CAPC data in positional clone, but also developed about 30 novel makers based on CAPC and new protocol of heterogeneous double helix analysis for SNPs or small InDels. 我们不仅利用CAPC数据资源进行定位,而且还发展了近30个新分子标记,在分子标记的检测方面发展了异源双螺旋分析技术。
- As the most efficient strategy in gene clone, positional cloning has been used widely in QTL cloning in rice. 图位克隆作为基因克隆的最有效手段, 在水稻QTL克隆方面取得了很大的进展。
- Thin recombinant with the cDNA ~crt fragment was released frc the positive clone and was di ?z; tcJ with EcoRI; the eDNA fra, ent was 2kb in length. 释放质粒后，进行酶切鉴定，该阳性克隆cDNA插入片段的大小为2kb左右。
- Blast analysis displayed one of the Northern blot positive clone is homologous with autotaxin gene, and another is homologous with calcyclin gene. 对这13个克隆进行测序和Blast同源序列分析表明，其中一个Northern杂交阳性克隆与autotaxin基因同源，另一个与钙周期蛋白基因同源。
- Methods:Screening peptide library with EAN serum. Identified positive clone by ELISA and compared similarity between positive clone and LPS of Campylobacter jejuni. 方法 :采用噬菌体呈现技术对 8个EAN模型血清抗体进行肽库筛选 ,并将获得的克隆用ELISA方法进行阳性鉴定 ,以及将阳性克隆与空肠弯曲菌LPS相似性进行比较。
- Otherwise, the nodus of QTL positional cloning and the corresponding solving methods were discussed. 文章还对QTL图位克隆的难点及解决方法进行了有益的探讨。
- Zhang YY, Proenca R, Maffei M, et al. Positional cloning of the mouse obese gene and it's human homdogue[J]. Nature,1994, 372,425-432. 汪丽惠，许广润，张树基，等．现代内科诊疗手册[M]．北京医科大学、中国协和医科大学联合出版社，1994．507
- Objective To investigate the causative genes of the kindred with genetic hearing impairment by means of positional cloning approach. 摘要目的探讨遗传性聋基因的定位克隆研究。
- The recombinant plasmid was transformed into the yeast strain S. cerevisiae BJ1990 to carry out the primary expression experiment. In culture supernant of screened positive clone, the hirudin activity was detected to be 30 ATU/ml. 用正确的重组表达质粒转入酿酒酵母BJ1990细胞进行了初步表达实验，在筛选出的阳性克隆的培养上清中检测出的水蛭素活性为30ATU/ml，表达量达4mg/L。
- MBD1 siRNAs had beensuccessfully integrated into the plasmid. MBD1 siRNAs vector was transfected intopancreatic cancer cell AsPC-1 by liposome. Positive clone was obtained by the screenof G-418. 采用脂 质 体 介 导 的 方 法 将 MBD1siRNAs 表 达 质 粒 转 染 胰 腺 癌 细 胞 系AsPC-1,通过 G-418 的筛选获得稳定表达 MBD1siRNAs 的阳性克隆。
- In this study, the dicistronic expression vector (pEGFP-C1) was used to be transfected into human lung cancer cell line(ASTC-a-1)and a positive clone which stably expressed GFP in high level was obtained. 利用常规转染方法将带有EGFP基因的质粒载体导入人肺腺癌肿瘤细胞 (ASTC a 1) ,并得到GFP稳定表达的细胞株。
- The obtained bands were cloned into pGEM T-Easy vector, and the positive clone were confirmed by EcoR I enzyme digestion. 将所获片段克隆入pGEN T-Easy载体，EcoR I酶切鉴定。
- QTL mapping opens up broad prospects for marker-assisted selection of complex quantitative disease resistance characters and the positional cloning of quantitative resistance genes. 展望了QTL作图对复杂的数量抗病性的标记辅助选育和数量抗性基因图位克隆的发展前景。
- Positional Clone and Genetic Analysis of ess Mutant of Arabidopsis Thaliana 拟南芥ess突变体定位克隆及遗传分析
- AL01 library contained 23 650 positive clones and the av-erage foreign DNA fragments were about 3.2 kb. AL01文库包含23650个克隆;随机插入载体的外源DNA 片段平均大小为3.;2Kb 左右;DNA 文库的总容量为75
- The obtained 30 positive clones were all sequenced, and analyzed by BLAST （basic local alignment search tool）. 将得到的30个阳性克隆全部进行测序，并进行BLAST同源序列比对分析。
- Conclusion The identification of positive clones provides a possible way for the development of toxoplasmosis vaccine. 结论阳性克隆的筛选和鉴定为抗弓形虫病疫苗的研制提供又一途径。
- SPV Vp2 protein was successfully expressed by the positive clones under the induction of IPTG. （2）首先用大肠杆菌表达和部分纯化的SPV VP2融合蛋白建立的Western印迹分析检测了猴子暴露者中的血清SPV抗体，同时用检测人细小病毒B19 IgG抗体的商品试剂检测细小病毒抗体以排除可能存在的B19的交叉抗体反应。
- Wait for foster mother to give birth to the clone. 等待“养母”产下克隆幼仔。
- Will 2003 be the year of the first human clone? 人类第一个克隆人会在2003年诞生吗?
