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- Methods DNA sequencing of region encoded HIV?1 SF?2env gene near PL promotor in recombinant prokaryotic plasmid was completed by ABI Automatic DNA Seq Machine. 方法使用ABI公司的373A自动测序仪，对插入有HIV－1SF2株env基因的原核表达质粒pSF2env近启动子DNA序列进行了序列测定。
- The enzyme restriction sites were added at the head of ATG of the two genes considering the express of the prokaryotic plasmid pET-28a(+) and the eukaryotic plasmids pcDNA3.1(+) and pEGFP-C3 when designing the primers of the two genes. 引物设计时，根据原核表达载体pET-28a(+)和真核表达载体pcDNA3.;1(+)、pEGFP-C3表达的需要，在基因起始密码子ATG前加入酶切位点的同时，注意补加碱基，以确保不发生读码框移。
- prokaryotic plasmid 原核质粒
- AIM: To construct pET32a/His MBL-CLR recombinant prokaryotic expression plasmid and to express mannan-binding lectin-CLR (MBL-CLR) protein in E. 目的:在大肠杆菌中表达人甘露聚糖结合凝集素(MBL)胶原样区(CLR)蛋白。
- Methods:The epf gene fragment was amplified by PCR from ZYH24 and cloned into prokaryotic expression plasmid pGEX4T-2 to form pGEX4T-2-epf. 方法根据GenBank S.;suis2epf基因序列设计引物;克隆ZYH24株epf基因片段并进行序列分析;
- Then the BLY cDNA fragment was subcloned into prokaryotic expression vector pGEX-4T-1, forming the prokaryotic expression plasmid (pG-BLY). 克隆PCR产物，并构建了pGEX-4T-1-BLY原核表达载体。 经BamHI和EcoRI酶切及质粒PCR鉴定，证实本实验构建的新型牛溶菌酶基因已克隆到原核表达载体pGEX-4T-1上，为进一步研究其诱导表达条件及生物学功能奠定了基础。
- Objective To construct the prokaryotic expression plasmid expressing woodchuck hepatitis virus core antigen(WHcAg) and prepare polyclonal antibodies. 目的构建土拨鼠肝炎病毒核心蛋白质粒并进行原核表达、抗体制备。
- The plasmid pBV220-Arr. could be expressed in E. 成功构建的重组质粒pBV220-Arr在E.
- Sex may also be derived from prokaryotic processes. 性别也可能是源于原核进程。
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- The choEW was inserted into prokaryotic expression vector pET-His, and the resulting recombinant plasmid pETW was used to transform E. colt BL21(DE3)plysS. 将choEW插入到原核表达载体pET-His中,构建出重组质粒pETW,并转化Escherichia coli BL21(DE3)plysS获得工程菌。
- Methods:(CTP)_4coding gene from pBSMR-(CTP)_4 was cloned into prokaryotic expression vector pET-28a(+) and the expression plasmid pET-28a(+)- (CTP)_4was obtained. 方法:将pBSMR-(CTP)_4中的(CTP)_4基因片断克隆入表达载体pET-28a(+),得到表达质粒pET-28a(+)-(CTP)_4。
- The identified cDNA was cloned into the new type of prokaryotic expression vector pQE-80L, and a synthetized expression plasmid named pQE-80L/DHFR/ABP obtained. The E. 将此基因克隆到原核表达载体pQE-80L,获得融合表达质粒pQE-80L/DHFR/ABP,在1%25IPTG诱导下进行表达。
- All living cells can be classified as prokaryotic or eukaryotic. 所有活细胞可以被划分为原核和真核两类。
- Recombinant plasmid pGEX-Csn was transformed into E. 重组质粒pGEX-Csn转化E.
- They appear to have a mixture of prokaryotic and eukaryotic genes. 它们被认为是某些真核生物基因和原核生物基因的混合体。
- Objective To construct the plasmid expressing BDV p40 gene. 目的构建博尔纳病病毒p40基因重组表达质粒。
- In addition, a set of expression plasmid vectors, PMS-31b. 同时构建一组质粒表达载体PMS-31b。
- Constructed the expression plasmid pTrc-rCR and checked it. 构建表达质粒pTrc-rCR,并酶切检验;
- The PNL-BDNF-IRES2-EGFP plasmid was identified to be correct. 构建的慢病毒载体质粒PNL-BDNF-IRES2-EGFP经Sal I和BamH I双酶切鉴定正确,三质粒共转染293T细胞后荧光激发可见大量绿色荧光。
