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- The technology of LC ESI MS/MS for protein sequencing was briefly introduced. 本文对液相色谱与电喷雾电离串联质谱仪偶联 (LC ESI MS MS)技术分析蛋白质序列的方法进行了详细介绍。
- The preparation of derivatized amino polyp ropylene (APP) membrane by introducing aminophenyl group (AP) or isothiocyanate group (DITC) respectively for solid-phase protein sequencing is described. 采用氨基聚丙烯膜分别进行衍生引入氨苯基 (AP)和对苯二异硫氰酸基 (DITC)生成AP聚丙烯膜和DITC聚丙烯膜两种用于蛋白质固相序列测定的膜载体 .
- Sequencing results revealed that both DNA and amino acid sequences had no substantial homology to previously described DNA or protein sequences. 序列分析显示该克隆子的DNA和氨基酸序列与已知基因、蛋白质氨基酸均无同源性。
- De novo sequencing provides proteomics research work a new method to interpret tandem mass spectrum data without any help of protein sequence databases. 从头测序为蛋白质组研究提供了一种不用借助于任何蛋白质序列数据库信息,直接解读串联质谱数据的方法。
- Sequence of acidic bovine neuropeptide 1(ABNP 1)was carried out on PE ABI 491A Model Protein Sequencer. 用PE?ABI491A型蛋白测序仪对酸性牛神经肽1(ABNP?1)进行了测序研究。
- In fact, colinearity of DNA and protein sequences is thought to be a fundamental feature of the universal genetic code. 这项新研究表明蛋白质也有拼接现象,有时甚至产生的蛋白质片段或多肽以与亲本相反的方向拼接在一起。
- Encoding of protein sequence is a key issue in subcellular localization prediction. 摘要蛋白质序列的编码是亚细胞定位预测问题中的关键技术之一。
- Models of signal transmission - Bioinformatics is commonly understood as work with the DNA and protein sequences. 然而,数据库和计算分析也试图在这些序列可以广泛使用这前成为这一方面的另一个研究对象。
- The membrane strip holding the protein was subjected to automatic Edman degradation for sequencing of the N-terminal aminol acids using an Applied Biosystems 477A protein sequencer (Parkin Elmer). 可用在检测器出口通气泡和检测器入口注射有色样品的方法确认仪器正常和估计灵敏度。
- Integrated approach for manual evaluation of peptides identified by searching protein sequence databases with tandem mass spectra. 质谱鉴定的质量,需要仔细的人工校正。但费时费力,而且校正结果依赖于专家经验。本文用自动的方法进行。
- Protein two-dimensional gel electrophoresis maps databases distributes very separately referring to protein sequences databases. 相对蛋白质序列数据库而言 ,蛋白质双向凝胶电泳 ( Two-dimensional gel electrophoresis,2 DE)图谱数据库分布比较分散。
- Aim To clone the muscarinic receptors M1, M3 and M5 sequences of astrocyte cells,and compare the gene and protein sequences with those of neurons. 目的克隆胶质细胞M1、M3、M5受体亚型基因序列,比较胶质细胞和神经细胞M1、M3、M5受体亚型基因序列、蛋白质序列的差异。
- When a new gene is identified and its DNA sequence deciphered, it is still unclear what its corre onding protein sequence is. 当一个新基因被识别,其DNA序列被解读,人们仍旧无法搞清相应的蛋白序列是什麽。
- To investigate the occurrence of HGT in phages and their bacterial hosts, we have analyzed protein sequences of terminase across different species. 为了研究HGT在噬菌体及其宿主体内的发生情况,分析多个物种的终端酶蛋白质序列。
- When a new gene is identified and its DNA sequence deciphered, it is still unclear what its corresponding protein sequence is. 当一个新基因被识别,其DNA序列被解读,人们仍然无法搞清相应的蛋白序列是什麽。
- Another reported envelop protein, VP281 is found containing a cell attachment sequence by protein sequence analysis and may play a role in the virus attachment. 另一定位的囊膜蛋白VP281序列分析中含有一细胞吸附序列(cell attachment sequence),可能在病毒吸附中发挥作用。
- In order to attain the complete protein sequence of APLA2-1, this study had also determined the eDNA sequence by gene clone method and therefore deduced the protein sequence. 为了得到APLA_2-1的蛋白质全序列,本研究还利用基因克隆的方法测定了APLA_2-1的cDNA序列,从而推导出蛋白质序列。
- First, a fixed-dimensional feature vector is generated for each protein sequence using the frequency of the hydropathy blocks occurring in the sequence. 首先,利用蛋白质序列中亲水模块的出现频率,每条蛋白质序列被转换为一个特征向量。
- Then along came RNA editing, in which guide RNAs or enzyme action modify some messenger RNAs such that the final protein sequence cannot be deduced from the gene sequence alone. 但RNA编辑又来捣乱了:向导RNA和酶作用于信使RNA的修饰过程,这就使仅仅从基因序列来推断最终的蛋白序列成为不可能。
- The NCBI taxonomy database contains the names of all organisms that are represented in the genetic databases with at least one nucleotide or protein sequence. NCBI的分类学数据库包括所有遗传数据库里的生物体,这些数据至少含有一种核苷酸或蛋白质序列。