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- Establishment and application of quantitative real time PCR assay for detecting duck viral enteritis virus 鸭病毒性肠炎病毒荧光实时定量PCR检测方法的建立和应用
- Keywords Root of Mallotus Apelta （Lour.） Muelle.-Arg.;Duck Hepatitis B Virus;Drug effects;Quantitative Real time PCR;Pharmacology; 中药;白背叶根;鸭乙肝病毒;实时荧光定量PCR;药理;
- Keywords gene diagnosis;fluorescent quantitative real time PCR;spinal muscular atrophy （SMA）;Carrier;light-weight directory access protocol （LDAP）;hospital information system; 基因诊断;荧光定量实时PCR;脊髓性肌萎缩;携带者;轻量级目录访问协议;医院信息系统;
- quantitative real time PCR 实时定量PCR
- Methods Quantitative detection of T cell receptor excision DNA circles (TRECs) in DNA of peripheral blood mononuclear cells from 8 cases with CML, CD4 + or CD8 + cells from 3 cases with CML were preformed by real time PCR (TaqMan) analysis. 方法 利用实时定量PCR(Taq Man)方法检测 8例CML患者外周血单个核细胞、3例CML患者CD4 + 和CD8+ 细胞中TRECs的水平。
- Use RT-PCR and real time PCR to quantify the HLA-G mRNA on deciduas. RT-PCR和real time PCR法检测胎盘蜕膜组织HLA-G mRNA。
- The plasma biochemical indexes were detected by using the kit, and real time PCR was used to analyze the expression of adipogenesis genes with time series. 试剂盒检测血脂生化指标，荧光定量 PCR 技术检测聚脂相关基因的转录表达规律。
- Totally 240 throat swab specimens collected from adults with flu like symptoms were detected both by the multiplex real time PCR system and conventional virus isolation. 我们收集240名成人具有类流感症状的咽喉拭子检体，利用此技术进行病毒筛检，并与病毒培养结果比较。
- The population of M. formicicum by real time PCR was lower than the population of M. formicicum by the method of most probable number, and the positive correlation between them was very high(r=0.957. P<0.01). M.;formicicum采用Real Time PCR方法测得的数量低于采用传统最大或然数法测得的数量;但两者之间具有很高的相关性(r=0
- Methods A549 cells were treated with different cytotoxic drugs for 24hours. The changes of APE/ref-1 expression in A549 after drug-treatment were detected by real time PCR, western blot and streptavidin peroxidase immunohistochemistry staining. 方法 通过免疫组化染色明确APE/ref-1在A549细胞中的表达后,以特定浓度的临床常用细胞毒性药物对A549细胞进行诱导,采用实时荧光定量PCR、Western Blot和免疫组化技术检测药物诱导前后A549细胞内APE/ref-1表达的变化情况。
- relative quantitative real time RT-PCR 荧光实时相对定量RT-PCR
- Genehunter analysis is concordant with ETDT. By real time PCR, OAZ(OLF1/ EBF associated zinc finger protein)gene which is located in the positive locus showed higher expression in SLE patients than in normal controls. 对候选基因的研究显示 OAZ(OL F1/ EBF- associated zinc finger protein)基因表达显著高于正常对照。
- The human colon cancer LoVo cells were transfected by Lipofectamine 2000 with plasmid vector, and then selected for 4 weeks by G418. The expression of cerb-B1 mRNA was assessed by real time PCR and the protein of EGFR was assessed by Western blot method. 应用脂质体Lipofectamine2000转染人结肠癌LoVo细胞,转染成功后以G418筛选4周,实时荧光定量RT-PCR(RealTimeRT-PCR)检测cerb-B1基因的mRNA表达,Western-blot检测EGFR蛋白的表达。
- RT-PCR, real time PCR or NASBA are used to pathogen test on cloacal or throat swab and wild birds.If the conformity rate >70% for HI antibody, it can be certified as conformity of vaccination. 疫情监测用RT-PCR或荧光定量PCR或NASBA(以来核酸序列的扩增技术)等方法进行泄殖腔拭子、咽喉拭子和野鸟粪便的病原学检测。
- In order to to detect Penicillium expansum rapidly in apple juice concentration,the real time PCR method was used to detect Penicillium expansum and the conditions were also optimized. 为了研究苹果汁生产中棒曲霉素产生菌的快速检测,利用实时PCR方法快速检测扩展青霉的可能性,对扩增条件进行优化。
- Lee, D. S., “Reserch and Development of a Novel Real Time PCR Machine,” Department of Mechanical Engineering, National Taiwan University, Dissertation for Doctor of Philosophy, 2004. 吴孟勋，新型复式即时定量聚合酶连锁反应检测机台开发与研究，国立台湾大学机械工程研究所硕士论文，台北，2004。
- Real time PCR quantification methods 实时定量法
- Keywords hepatitis B virus;real time PCR;HBeAg; 乙型肝炎病毒;聚合酶链反应;荧光定量;
- Keywords Real Time PCR;virus;Inactivation and removal efficiency; 实时荧光定量PCR;病毒;灭活去除效率;
- Methods A real time quantitative PCR(RQ-PCR) was performed to measure the bcr/abl~(P190) transcripts in the bone marrow mononuclear cells from 74 CML patients in different phases. 方法应用实时定量聚合酶链反应(RQ-PCR)检测74例CML患者骨髓单个核细胞中的bcr/ablP190转录本。
