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- Establishment and application of quantitative real time PCR assay for detecting duck viral enteritis virus 鸭病毒性肠炎病毒荧光实时定量PCR检测方法的建立和应用
- Keywords Root of Mallotus Apelta （Lour.） Muelle.-Arg.;Duck Hepatitis B Virus;Drug effects;Quantitative Real time PCR;Pharmacology; 中药;白背叶根;鸭乙肝病毒;实时荧光定量PCR;药理;
- real - time RT PCR 荧光定量RT-PCR
- relative quantitative real time RT-PCR 荧光实时相对定量RT-PCR
- Keywords gene diagnosis;fluorescent quantitative real time PCR;spinal muscular atrophy （SMA）;Carrier;light-weight directory access protocol （LDAP）;hospital information system; 基因诊断;荧光定量实时PCR;脊髓性肌萎缩;携带者;轻量级目录访问协议;医院信息系统;
- Real time RT - PCR 实时荧光RT-PCR
- quantitative real time PCR 实时定量PCR
- Objective To construct and evaluate the competitive quantitative RT PCR method for detecting the fusion gene of AML1 ETO in the patients of t(8;21)AML(acute myeloid leukemia). 目的 建立针对 AML- ETO融合基因转录本的竞争性定量反向 -聚合酶链反应 (RT- PCR)体系 ,并探讨其在 t(8;2 1)急性髓细胞性白血病 (acute myeloid leukemia,AML)微小残留病变跟踪中的应用价值。
- RT PCR was performed for APN gene expression in liver tissue. (2 )APN表达 :根据兔APN基因cDNA序列设计引物 ,提取肝总RNA ,利用RT PCR方法 ,检测肝APNmRNA水平的变化。
- Methods A real time quantitative PCR(RQ-PCR) was performed to measure the bcr/abl~(P190) transcripts in the bone marrow mononuclear cells from 74 CML patients in different phases. 方法应用实时定量聚合酶链反应(RQ-PCR)检测74例CML患者骨髓单个核细胞中的bcr/ablP190转录本。
- Methods Plasma ETA was measured quantitively by RT PCR. 方法 应用 RT-PCR技术检测外周血循环中 ETA基因表达水平。
- Methods The expression of MDR1 gene in 77 cases of AL patients and 10 cases of control group was detected with semi quantitative reverse transcription polymerase chain reaction (RT PCR). 方法 采用半定量逆转录聚合酶链反应（ R T? P C R），对77 例 A L病人及 10 例正常人 M D R1 基因表达进行检测。
- The mRNA levels of this gene were determined in SCLC cell line SH77 ,big cell lung cancer cell line H460,lung adenocarcinoma cell line SPC A 1 and SPC A 1/CDDP using semi quantitative RT PCR assay. 应用半定量RT-PCR方法检验小细胞肺癌SH77、大细胞肺癌H460、肺腺癌SPC-A-1和SPC-A-1/cDDP等细胞株中该基因mRNA的表达。
- Methods Quantitative detection of T cell receptor excision DNA circles (TRECs) in DNA of peripheral blood mononuclear cells from 8 cases with CML, CD4 + or CD8 + cells from 3 cases with CML were preformed by real time PCR (TaqMan) analysis. 方法 利用实时定量PCR(Taq Man)方法检测 8例CML患者外周血单个核细胞、3例CML患者CD4 + 和CD8+ 细胞中TRECs的水平。
- Angiotensin receptor (ATR) mRNA expression of VSMC was measured by RT PCR. RT PCR检测VSMC血管紧张素受体 (ATR)mRNA表达。
- Expression of the M 2-1 gene was examined by RT PCR and Western blot. 重组真核质粒PXJ 4 1/M2 -1转染肺腺癌PAa细胞 ,应用RT PCR、Westernblot方法检测表达。
- Use RT-PCR and real time PCR to quantify the HLA-G mRNA on deciduas. RT-PCR和real time PCR法检测胎盘蜕膜组织HLA-G mRNA。
- The expression of cyclin D1 and CDK4 were detected by RT PCR method. RT PCR检测细胞周期素D1(cyclinD1)、细胞周期蛋白依赖激酶 4 (CDK4 )mR NA的表达。
- This will be very quick. It will be real time. 它将是非常快捷和实时的。
- Windows NT can be used in real time systems. 窗口新台币能在真正的时间系统中被用。
