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- The adrenoreceptor density of peripheral lymphocyteswere measured by radio ligand binding assay,and the content of interleukin-5(IL-5) and interleukin-12(IL-12) were measured by enzyme-linked immunosorbnent assay. 用放射性配基结合分析法测定外周血淋巴细胞受体的变化,测定血清白介素5(interleukin-5,IL-5)、白介素12(interleukin-12,IL-12)含量。
- receptor radio ligand binding assay 受体放射配体结合分析
- radioactive ligand binding assay 放射性配基结合分析
- Keywords LHRH-PE40;radioactive ligand binding assay;affinity; 放射性配基结合分析;亲和力;
- Pressure Effects on Ligand Binding Kinetics for Hemoproteins. 压力对血红素蛋白配合基键联动力学的影响。
- In PMP radio binding assay of two different 3HPAF concentrations,each specific binding inhibition effect of SY were dosage depenent also. 两种浓度3HPAF作为配体进行PMP受体结合实验,不同浓度的红花总黄色素抑制3HPAF与受体的特异性结合均可见明显的量效关系。
- RESULTS In WRP radio binding assay of three different 3HPAF concentrations,each specific binding inhibition effect of SY were dosage dependent. 结果3种浓度3HPAF作为配体进行WRP受体结合实验,不同浓度的红花总黄色素抑制3HPAF与受体的特异性结合均可见明显的量效关系;
- OBJECTIVE In order to clarify whether the Safflor Yellow (SY) is a new platelet activating factor antagonist,we adopted the radio binding assay of SY prepared through silica adsorption procedure. 目的观察硅胶吸附法制得的红花总黄色素体外对氚标记血小板激活因子(3HPAF)血小板受体结合的影响,试图证明该药为一新型血小板激活因子(PAF)受体拮抗剂。
- Consequent upon the structural flexibility and the ligand binding, circulating albumin exhibits chemical and physical microheterogeneity. 由于其结构上的易变性和配体结合的不同,循环中的白蛋白表现出化学和物理上的微观不均一性。
- Binding assay shown that Vp28 can not binding membrane protein of shrimp gill. 纯化的VP28与对虾鳃细胞膜的结合实验表明,VP28不能与对虾鳃细胞膜结合,即纯化的VP28不具有结合活性。
- METHODS: Guinea pig lung membrane was used as experimental materials, 3H LTC 4 as radio ligand, FPL 55712 as positive control drug and Gn 3 as test drug. 方法:以豚鼠肺膜为实验材料,采用3HLTC4为放射配体,以FPL55712作阳性对照药物,Gn3为实验药物,进行药物竞争结合实验。
- Some albumin molecules may hae undergone steric modification or ligand binding such that the immunoreactie sites hae become obscured. 某些白蛋白分子也许经历了空间修饰或配体结合上的改变以至于其免疫反应位点被湮没。
- The dopaminergic receptors were measured with the radio-ligand binding assay. 采用同位素标记法测定受体变化;
- Also the modeling presents the ligand binding site, active site and the putative binding of tRNA Trp . 模建结果对配基结合位点和活性位点以及可能与tRNATrp结合的方式也给出了预测。
- We need to further validate the relationship between the newly discovered cholesterol sites and the ligand binding sites on these receptors. 我们需要进一步地确认在这些受体中新发现的胆固醇位点和配体位点的关系。”
- Sequence differences in the ligand binding domain of PXR serve as the molecular basis for the species difference in CYP3A induction. 化学物通过PXR调节CYP3A的表达可能是影响化学物体内代谢的一条重要途径。
- From the calculations, it can be found that the van der Waals interactions, the hydrophobic interactions, as well as the H-bonding interactions are crucial for ligand binding. 计算得到的抑制剂和靶酶之间的相互用模式显示范德华相互作用、疏水相互作用以及氢键作用是主要的作用模式。
- Competitive protein binding assay indicates the level of cAMP is improved when CDCA is given by oral administration. 通过竞争性蛋白质结合分析法测得,鹅去氧胆酸经灌胃给药后能显著提高大白鼠气管组织中cAMP的含量。
- SSR was detected by radioligand binding assay(RBA) and ER, PR by LsAB method in these groups. 采用RBA法测定各组织的SSR表达 ,采用免疫LsAB法测定ER、PR表达。
- The effects of l-CSL on recombinant D_1R were investigated by [3~H]SCH23390 binding assay and cAMP assay. [~3H]SCH23390受体结合和cAMP测定实验检测左旋氯代斯阔任对重组D_1受体的激动作用。