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- Competing ELISA showed that recombinant ScFv could compete with antiserum to specific hind BoNTa. 竞争活性测定结果表明,重组抗毒素在体外具有良好的活性,可竞争肉毒抗毒素马血清与毒素的特异结合。
- coli BL21star (DE3) and expression of the recombinant scFv gene was induced by addition of 0.2 mM IPTG. 经酶切鉴定和序列测定后,将pET-scFv转化入大肠杆菌BL21star(DE3)中,进行诱导表达。
- recombinant scFv 重组单链抗体
- Results A recombinant phage ScFv library with liter of 4X109 pfu/ml was established. 结果 用M13KO7援救XL-Blue转化菌中的pCANTAB5E重组噬菌体,得到滴度为4×10~9 pfu/ml噬菌体单链抗体库;
- Results The anti-carcinoma ScFv immunotox recombinant plasmid expressed the ScFv immunotoxin in inclusion.Its molecular weight was about 66KD. 结果诱导表达了约66KD的蛋白,分子量符合预计大小,以包涵体的形式存在于菌体中。
- To obtain the anti-transferrin receptor scFv and streptavidin(SA) recombinant fusion proteins which were high-efficiently and solubly expressed in E. 拟获得在大肠杆菌中能高效可溶表达的转铁蛋白受体单链抗体与链亲和素(SA)的重组融合蛋白。
- Phage antibody chips, prepared by coupling the recombinant phage displaying scFv with carboxyl-terminated silicon, can be applied in detecting proteins and proteomes. 摘要将展示单链抗体的重组噬菌体与羧基终止的矽片偶联,制成噬菌体抗体芯片,可用于检测多类蛋白质和蛋白质组。
- Recombinant fragments;recombinant technology. 重组DNA碎片;重组DNA技术
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- Recombinant Hepatitis C Virus NS5?Genotype 1? 重组丙型肝炎病毒NS5?基因型1?
- However, the affinity of ScFv was lower than that of Fab. FV能特异性地与角蛋白结合而与其它抗原不结合,说明人源性抗角蛋白Fab抗体改建为SCFV后具有较好的特异性和亲和力,但可溶性表达的ScFv的亲和力低于可溶性Fab片段;
- Nesiritide is a recombinant human BNP (rhBNP). 奈西立肽是重组人脑钠肽。
- The weight of ScFv about 750bp after VH linked with VL by SOE PCR. 经重叠延伸PCR扩增,将vH和vL连接后构建的ScFv基因大约75obp;
- Purpose To identify recombinant human insulin and porcine insulin. 目的鉴别猪胰岛素与重组人胰岛素。
- Recombinant DNA is also an excellent example of this problem. 重组体dna也是这类问题的一个极好的例子。
- The Expression Analysis of Recombinant Protein in Escherichia coli. 大肠杆菌中表达外源重组蛋白的研究。
- Cloning, expression and purification of recombinant protein. 运用常规分子生物学与生化技术克隆表达与纯化蛋白质。
- Recombinant DNA techniques developed for cloning genes. 重组DNA技术被应用于基因克隆。
- Recombinant plasmid pGEX-Csn was transformed into E. 重组质粒pGEX-Csn转化E.
- The recombinant human lactoferrin could inhibit growth of E. 重组人乳铁蛋白具有抑制E.