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- The construction of recombinant adenoviral vector containing gene of EWS-FLI1 and its expression in PBMC . 介导尤文肉瘤融合基因的重组腺病毒的构建及其在外周血单个核细胞(PBMC)中的表达
- Objective Rabit bone marrow stromal cells(BMSC) infected by a recombinant adenoviral vector carrying the human BMP 2 gene(Ad BMP 2) were seeded into BCB scaffolds to construct tissue engineering bone in vitro. 目的 用人骨形态发生蛋白 2腺病毒表达载体 (Ad BMP 2 )转染的兔骨髓基质干细胞 (BMSC) ,种植BCB(去抗原牛松质骨 )支架体外构建组织工程骨。
- The effect of 12C6+ beam irradiation on AdCMV-GFP(a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated. 探讨12C6+离子束辐射对用带有绿色荧光蛋白基因的缺陷性腺病毒(AdCMV-GFP)转染小鼠黑色素瘤细胞(B16细胞系)的影响。
- AIM: To construct the recombinant adenoviral vector carrying antisense RNA to chemokine receptors CCR5 and CXCR4 and to obtain recombinant adenovirus, which will be used to resist HIV 1 infection. 目的 :构建趋化因子受体CCR5 ;CXCR4双靶区反义RNA重组载体并获取重组腺病毒以用于抗HIV 1基因治疗的研究 .
- RESULTS: Recombinant adenoviral vectors containing melittin gene were successfully constructed, and it was melittin gene that could be transcripted proved by RT-PCR. 结果：携蜂毒素基因的重组腺病毒载体构建成功，RT-PCR实验表明蜂毒素基因可以得到转录。
- Results: Adeno-X-SSTR2,recombinant adenovirus vector,can infect pancreatic carcinoma cells MIA-PaCa-2 and AsPC-1 efficiently,which both can express SSTR2 mRNA and their protein. 在不同浓度NC-8-12和奥曲肽作用下;未转染SSTR2基因胰腺癌细胞株的Survivin表达量无明显变化(P>0.;05);转染了SSTR2基因胰腺癌细胞株的Survivin表达量明显下调(P<0
- Abstract:Objective:To construct the recombinant adenovirus vector carrying rat vascular endothelial growth factor (VEGF) as preparation for later use for genetic transfection. 目的:探讨构建携带大鼠血管内皮细胞生长因子(VEGF)的重组腺病毒载体方法，为后续的基因转染研究作准备。
- Objctive:It is surveyed whether the recombinant adenovirus vector pAdeno-X-siFas 1+siFas 2 expressing two Fas-targeted short hairpin RNAs(shRNA) could supress the overexpression of Fas induced by LPS/D-GalN in BALB/c mouse liver. 目的:观察串联表达Fas-shRNA的腺病毒载体pAdeno-X-siFas 1+siFas 2对脂多糖(LPS)/D-氨基半乳糖(D-GalN)诱导BALB/c鼠肝细胞Fas过表达的影响。
- Successful construction of the recombinant adenovirus vector containing the Ki67-siRNA gene was achieved and it can be used to significantly inhibit expression of the Ki67 gene and the growth human colon carcinoma cells. 含有Ki67-siRNA的重组腺病毒构建成功,其能抑制人结肠癌细胞Ki67基因表达和生长。
- A study on the improved efficiency of extraction, purification and titration of recombinant adenoviral vector 提高重组腺病毒载体提纯和滴定效率的研究
- CONSTRUCTION OF ANTISENSE RECOMBINANT ADENOVIRAL VECTOR FOR c-myc AND IT'S ANTIPROLIFERATIVE EFFECT ON RAT LYMPHOCYTES 反义c-myc重组腺病毒载体的构建及其对大鼠胸腺淋巴细胞的增殖抑制作用
- Construction and purification of the recombinant adenoviral vector carrying antisense RNA to chemokine receptor CCR5 趋化因子受体CCR5反义RNA重组腺病毒载体的构建
- Construction of recombinant adenoviral vector containing gene of EWS-FLI1 and antitumor immunity of its modified dentritic cell in vitro 尤文肉瘤融合基因修饰的重组腺病毒的构建及其转染的树突细胞体外抗尤文肉瘤免疫应答
- recombinant adenoviral vector 重组腺病毒载体
- The recombinant adenovirus vector, pAdCMV-IL3-Endo, was cotransfected into 293 cells together with pBHG10 by lipofectamine, and recombinant adenovirus, which named Ad-ILs-Endo, were generated by homologous recombination. 然后用脂质体法将上述穿梭质粒与质粒pBHG10共转染293细胞，经同源重组获得复制缺陷型重组腺病毒Ad-IL_3-Endo。
- Keywords recombinant adenovirus vector;gene therapy;safety; 腺病毒载体;基因治疗;安全性;
- Preparation and identification of recombinant adenoviral vectors containing human wild type SPK and its mutant gene 携带人鞘氨醇激酶及突变体基因重组腺病毒载体的制备及表达
- The Propagation and Purification of Human HOSM Recombinant Adenovirus Vector 重组腺病毒载体含人抑瘤素的扩增及纯化
- The Construction and Identification of Recombinant Adenovirus Vector of hIL-24 人白介素24腺病毒载体的构建与鉴定
- Construction of recombinant adenovirus vector expressing the human IL-10 人白细胞介素10重组腺病毒载体的构建
