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Cloning of ACC Oxidase Gene of Peach and Construction of Its Plant Recombinant Expression Vectors?? 桃ACC氧化酶基因的克隆和植物表达载体的构建
Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
Objective: To construct the recombinant expression vector of genes encoding light chain of antibody against Mumps Viruses. 目的:构建抗腮腺炎病毒抗体轻链基因重组表达载体。
Objective To review the recombinant expression systems of calcitonin and provide a reference for the further research and development of calcitonin. 目的综述基因工程法生产降钙素的研究进展,为进一步研究利用降钙素提供参考。
The results showed that eaeA gene was correctly inserted into the pET-28a(+) vector, and the recombinant expression plasmids (named pET-eaeA) were constructed successfully. 将经质粒PCR鉴定和双酶切鉴定为阳性的转化子测序作进一步鉴定，成功构建了重组表达质粒pET-eaeA。
A gene fragment of about 450 bp was amplified by PCR and the recombinant expression vector pGEX4T1-CRD was constructed, whose restriction maps and sequence were consistent with those of expected one. PCR扩增得到长约450bp的目的基因片段,插入pGEX4T1载体所获重组表达载体pGEX4T1CRD的酶切图谱和序列与预期的一致。
Subsequently, the P36 gene of Mhp strain ZCF23 was subcloned into the prokaryotic expression vector pGEX 6P-1 through EcoR I and Xho I double digestion, then the recombinant expression vector pGEX 6P-1-P36 was constructed and transformed into E. 序列确认后，用Ec口Rl和肠口I将P36基因从克隆载体中切出，并插入到GsT融和表达载体pGEx 6p一1相应位点，构建成重组表达载体pGEX 6p一1一P36，并转化宿主菌BLZI。阳性克隆命名为BLZI(6P一l一P36)。
Restriction endonuclease digestive identification was right for recombinant expression vector pCD11b BFP. Blue fluorescence from fusion protein could be seen in U937 cells transfected with plasmid pCD11b BFP. 经酶切鉴定 ,p CD11b- BFP构建完全正确 ,转染 U937细胞株后 ,可见 CD11b- BFP融合蛋白发出的蓝色荧光。
The sequences coding two target proteins were cloned into the above recombinant expression plasmids separately and transfected into Cos 7 cells mediated via liposome. The expression products were detected by Western blot and co immunoprecipitation. 将两种候选蛋白的编码序列分别克隆于上述融合载体,转染Cos?7细胞后的表达产物,可利用抗HA或myc的mAb进行Western?blot和免疫共沉淀。
The expression vector of humanized antibody light chain gene of against HCV is constructed effectively which laya solid foundation for further constructing the recombinant expression vector of humanized antibody Fab fragment against HCV. 人源性丙肝病毒抗体轻链基因表达载体是高效的,为下一步构建丙肝病毒抗体F ab段基因重组载体奠定了基础。
Methods Recombinant expression vectors containing CD (cytosine deaminase)and/or TK(thymidine kinase) gene under CMV promoter were constructed successfully. The vectors were transfected to GLC-82 tumor cell lines by use of lipofectamine. 方法分别构建了以 CMV为启动子 ;含单纯疱疹病毒胸苷激酶（ HSV-TK）和 /或大肠杆菌胞嘧啶脱氨酶（ Ecoli.;CD）单、双自杀基因的真核表达载体。
The GAP-43 gene was inserted into eukaryotic expression vector pEGFP-N3.The recombinant expression vector was transiently transfected into COS-7 cells by LipofectamineTM 2000 reagent. 以LipofectamineTM2000试剂转染pEGFP-N3-GAP-43表达载体至COS-7细胞中进行瞬时真核表达。
Methods:The full-length gene of NY-ESO-1 was generated by gene splicing method and the recombinant expression vector NY-ESO-1-pET-28a (+) was constructed. E. coli BL21 (DE3) bearing the plasmid was induced with IPTG for protein production. 方法:通过全基因拼接获得NY-ESO-1基因,构建重组表达载体NY-ESO-1-pET28a(+),在大肠杆菌BL21(DE3)中利用IPTG诱导获得表达,利用单克隆抗体进行Western印迹鉴定,通过Ni柱亲和纯化获得纯化蛋白。
The whole FC cDNA was cloned into pET-28a (+) containing T7 promoter and recombinant expression plasmid pET-FC was constructed. The recombinant plasmid was transformed into E. coli BL21(DE3). 将分段克隆得到的两段FC片段经相应酶切后 ,与含T7启动子的质粒 pET 2 8a(+)在一个体系中作连接反应 ,构建表达质粒pET2 8a FC ,转化大肠杆菌BL2 1(DE3) ,筛选表达菌株。
The mRNA of broiler HSP70 gene was amplified by RT-PCR and the production was cloned into the PGEM-T Easy vector and the expression vector pET-28a (+) . The recombinant expression vector was transformed into E. coli BL-21 and induced by IPTG to express. 利用RT-PCR方法扩增了肉鸡HSP70 mRNA,将其扩增产物克隆到PGEM-T Easy载体和原核表达载体pET-28a(+)上,进一步转化至大肠杆菌BL-21中,用IPTG诱导表达重组肉鸡HSP70。
The result of immunologic test showed that there was crossed protection between the two bacteria.Vanhnaseng(2004) cloned the gene of cIL-18 and obtained recombinant expression plasmid pcDNA3.1/cIL-18. 曹素芳（2004）克隆了禽巴氏杆菌外膜蛋白H基因（ompH），构建了该基因与cIL-18基因真核共表达质粒pcDNA3.;1/ompH-cIL-18，免疫试验结果表明，pcDNA3
The recombinant expressing plasimd of psectagB/His-myc-IL29 was constructed by inserting IL-29 cDNA into the vector and was then transfected into cos-7 cells. DNA测序正确后,将IL-29 cDNA的编码框序列构建到真核表达载体psectagB/His-myc中。
An expression of pain flitted across her face. 她脸上闪过一种痛苦的表情。
GAD67 gene had been cloned from rat brian using RT-PCR and T vector techniques. The success of cloning of Glutamic acid decarboxylase 67 gene lays a good basis for construction of a recombinant expressed. 采用RT -PCR和T载体技术获得了大鼠脑组织中的谷氨酸脱羧酶GAD6 7基因克隆 ;为该基因的体外表达打下基础 .
He always shows a doleful expression. 他总是露出忧郁的表情。