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- Methods: A recombinant cosmid containing CD and TK fusion gene was constructed, and then mixed with DNA TPC and co transfected to the 293 cells by calcium phosphate coprecipitation. 方法:构建含有CD、TK融合基因的重组粘粒,将其与腺病毒DNA末端肽复合物混合后以磷酸钙共沉淀法转染人胚肾293细胞株细胞,获取重组腺病毒,并进行鉴定。
- recombinant cosmid 重组黏粒
- General purpose cosmid vector derived from pBR322. 相关说明：vector for constructing cDNA libraries；
- Recombinant fragments;recombinant technology. 重组DNA碎片;重组DNA技术
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- Recombinant Hepatitis C Virus NS5?Genotype 1? 重组丙型肝炎病毒NS5?基因型1?
- Nesiritide is a recombinant human BNP (rhBNP). 奈西立肽是重组人脑钠肽。
- A set of cosmid clones were obtained from cosmid library using DNA prob from... 以pcos2EMBL为载体构建了cosmid分子克隆库。并用果蝇的与生物节律控制...
- Purpose To identify recombinant human insulin and porcine insulin. 目的鉴别猪胰岛素与重组人胰岛素。
- Recombinant DNA is also an excellent example of this problem. 重组体dna也是这类问题的一个极好的例子。
- The Expression Analysis of Recombinant Protein in Escherichia coli. 大肠杆菌中表达外源重组蛋白的研究。
- Cloning, expression and purification of recombinant protein. 运用常规分子生物学与生化技术克隆表达与纯化蛋白质。
- Recombinant DNA techniques developed for cloning genes. 重组DNA技术被应用于基因克隆。
- Recombinant plasmid pGEX-Csn was transformed into E. 重组质粒pGEX-Csn转化E.
- The recombinant human lactoferrin could inhibit growth of E. 重组人乳铁蛋白具有抑制E.
- It turns that most of the recombinant protein were inclusion body. 对重组蛋白的细胞内定位分析发现蛋白几乎全部以包涵体形式存在。
- Purpose:To give an appraisal of recombinant L-asparaginase. 目的:对重组L天冬酰胺酶进行鉴定。
- Methods: D116H-ANG - pAxCAwt cosmid DNA and DNA-TPC were mixed and co-transfected to the 293 cell by calcium phosphate coprecipitation. 方法：ANG衍生物D116H-ANG重组粘粒与腺病毒DNA末端肽复合物混合后以磷酸钙共沉淀法转染293人胚肾细胞株细胞。
- Through constructing genomic cosmid library of F. oxysporum f. sp. cubense race 4, two pathogenicity mutants, 865 and B1233 were studied further. 在构建香蕉枯萎病菌4号生理小种基因组cosmid文库的基础上，对致病突变体865和B1233进行了深入研究，获得致病相关基因的基因序列（包括外显子和内含子）；
- With porcine myoglobin genomic DNA fragment cloned in a cosmid as probe, the chromosome location of this gene was determined by fluorescent in situ hybridization (FISH). 摘要 利用荧光染色体原位杂交技术，以含有猪肌红蛋白基因的粘粒DNA为探针，分析了猪肌红蛋白基因在染色体上的物理位置。
