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- Methods The recombinant fusion protein MBP hEra was expressed in the E. 方法在大肠杆菌中,表达重组MBP?hEra融合蛋白。
- In terms of accine deelopment, we foresee some applications where it may be preferable to retain the Ig sequence on the final recombinant fusion protein. 我们预计，保留免疫球蛋白序列对融合抗原分子在疫苗研发上的应用是有利的。
- The plasmid pTWIN1/Sj-F1 was transformed into E. coli ER2566. The soluble recombinant fusion protein (rSj-F1/intein2) was expressed in E. coli under low IPTG concentration and low temperature, then analyzed by SDS-PAGE and Western blot. 将pTWIN1/Sj-F1质粒转化大肠杆菌ER2566;在低温和低IPTG浓度下诱导表达可溶性重组融合蛋白(rSj-F1/intein2);并经SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)和蛋白质印迹(Western blot)分析鉴定.
- Objective To compare the immunoenhancing effects between two adjuvants,Freund?s complete adjuvant(FCA) and Quil A(purified saponin),in mice vaccinated by recombinant fusion protein SjGST-32(rGST-Sj32). 目的 比较弗氏完全佐剂 (FCA)与皂素的纯化物 Quil A的免疫增强作用 ,进一步提高重组抗原 r GST- Sj32的免疫效果。
- Abstract: Recombinant fusion protein staphylokinase-hirudin has been produced by adopting the dissolved oxygen feed-back fed-batch with the high cell density cultivation strategy in E.coli BL21(DE3). 摘要: 采用溶氧反馈的分批培养流加补料的方法高密度培养重组大肠杆菌BL21(DE3)生产重组葡激酶-水蛭素融合蛋白。
- To obtain the anti-transferrin receptor scFv and streptavidin(SA) recombinant fusion proteins which were high-efficiently and solubly expressed in E. 拟获得在大肠杆菌中能高效可溶表达的转铁蛋白受体单链抗体与链亲和素(SA)的重组融合蛋白。
- BO-E2 recombinant fusion protein BO-E2融合蛋白
- Ag85A recombinant fusion protein Ag85A重组蛋白
- recombination fusion protein 融合蛋白
- Keywords Granulysin;Recombinant fusion protein;Western blotting; 颗粒溶素;重组融合蛋白;免疫印迹;
- Purified fusion protein was obtained. 得到纯化的融合蛋白。
- The recombination plasmid was transiently transfected into COS7 cells and the expression of the fusion protein was identified by RT PCR, immunofluorescent assay and sandwich ELISA. 以其瞬时转染COS7细胞 ,应用RT PCR、细胞免疫荧光技术 ,及夹心ELISA检测融合蛋白的表达。
- The GRA7 gene may be expressed as a GST fusion protein in E. coli. GRA7基因在大肠埃希菌中以GST融合蛋白的形式得到表达。
- COMPARISON BETWEEN FCA AND QUIL A IN IMMUNOPOTENTIATION OF RECOMBINANT FUSION PROTEIN GST-Sj32 VACCINATION IN MICE 佐剂FCA与Quil A对rGST-Sj32的免疫增强作用的比较
- Expression and immunogenesity analysis of a recombinant fusion protein of V.Cholera ctB and H.pylori ure I ctB和ure I双基因融合表达及其免疫特性分析
- The Recombinant Fusion Protein of CTB and HCV Epitope: Antigenicity and Application to Anti-HCV ELISA Kit CTB与HCV抗原决定簇融合蛋白的抗原性及其在抗HCV ELISA试剂盒中的应用
- Preliminary Study on the Expression of Survivin and the Combination Therapy with It's Antisense Gene and Recombinant Fusion Protein of Bladder Cancer 在膀胱癌的表达及其反义基因联合重组融合蛋白治疗膀胱癌的实验研究
- The fusion protein was purified by GSTrap FF affinity chromatography. GSTrap FF亲和层析柱纯化融合蛋白;
- The fusion protein was purified through Nickel-affinity chromatography column. 用镍柱纯化融合蛋白。
- Expression, purification and bioactivity of a recombinant fusion protein consisting of anti-HBs single chain Fv and interleukin-2 重组人抗-HBs单链抗体与白细胞介素2融合蛋白的表达、纯化及活性鉴定
