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- PCR analysis and histochemical assay of GUS activity are conducted to testify the transformation events and the successful expression of reporter gene in independent hygromycin resistant calli. 为建立银杏遗传转化的高效体系,对一些重要的影响银杏转化的因素,包括外植体类型,选择标记和筛选压,农杆菌工程菌株,共培养时间和恢复时间第一次进行了试验并优化了条件。
- Co-transformation with Agrobacterium strains AGL1 and EHA 105, and by re-suspending the strains with NB culture media before transformation could improve the resistant calli rate significantly. 结果表明,菌株AGL1和EHA105按一定比例混合共转化和转化前菌体重悬对抗性愈伤率有显著影响;
- Media for resistant callus: B5+ 0.1 mg/L 2, 4-D + 0.2 mg/L KT + 75 mg/L Kan +500 mg/L Cef. Media for resistant shoots: B5 + 100 mg/L Kan + 500 mg/L Cef. 获得抗性愈伤培养基:B5+0.;1mg/L2;4-D+0
- Growth Law of Rice Resistant Calli Transformed by Particle Bombardment 水稻基因枪转化过程中抗性愈伤的生长规律
- The nobility usually resist social changes. 贵族通常反对社会变革。
- They are determined to resist invasion. 他们决定抵抗入侵。
- I can never resist an ice cream. 我一见到冰淇淋就忍不住想吃。
- He could not resist the temptation to steal. 他经不起想要偏巧的诱惑。
- He couldn't resist the lure of money. 他不能抵制金钱的诱惑。
- This special coating is designed to resist rust. 这一特别的涂层旨在抗锈。
- He was helpless to resist the temptation. 他无法抵御诱惑。
- If the enemy continue to resist, wipe them out. 如果敌人继续抵抗,就把他们消灭掉。
- The nation was unable to resist the invasion. 该国无力抵抗侵略。
- He can't resist the allure of the pretty waitress. 他无法抵抗漂亮的女招待的引诱。
- The establishment of blackwood calli system. 黑木相思愈伤组织体系的建立。
- Induction of calli from leaves ofA.philoxeroides;C. C.;从茎段和愈伤组织上形成的根 Fig
- Many young people could not resist the spiritual pollution. 很多年轻人不能抵制精神污染。
- Their opponents had the whip hand and it was useless to resist. 他们因对手权柄在握,反抗也没用。
- Resistant to authority or discipline; recalcitrant. 难驯服的不屈服于权力或权威的; 暴烈的
- Resistant to catching fire; flame-retardant. 防火的能防止着火的; 耐火的