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- Co-transformation with Agrobacterium strains AGL1 and EHA 105, and by re-suspending the strains with NB culture media before transformation could improve the resistant calli rate significantly. 结果表明,菌株AGL1和EHA105按一定比例混合共转化和转化前菌体重悬对抗性愈伤率有显著影响;
- resistant callus rate 抗性愈伤率
- The highest callus rate(81.0%) and regeneration rate(21.5%) were got on QL+TDZ 2.0 ?/L+NAA 0.75 ?/L+AgNO3 4mg/L medium. 在含TDZ 2.;0mg/L、NAA0
- Media for resistant callus: B5+ 0.1 mg/L 2, 4-D + 0.2 mg/L KT + 75 mg/L Kan +500 mg/L Cef. Media for resistant shoots: B5 + 100 mg/L Kan + 500 mg/L Cef. 获得抗性愈伤培养基:B5+0.;1mg/L2;4-D+0
- The effect of inducing callus of ?Aloe vera? L. on different media had been compared. In B?5 medium add ?2.0mg/L? 6?BA, 2.0mg/L NAA, 5mg/L Vc, The callus rate is high and the browning of callus is less. 比较了不同培养基对库拉索芦荟愈伤组织的诱导效果;其中B5+6 BA2.;0mg/L+2;4 D2
- At this rate we won't be able to afford a holiday. 照这样下去,我们不会有时间/钱去度假的。
- PCR analysis and histochemical assay of GUS activity are conducted to testify the transformation events and the successful expression of reporter gene in independent hygromycin resistant calli. 为建立银杏遗传转化的高效体系,对一些重要的影响银杏转化的因素,包括外植体类型,选择标记和筛选压,农杆菌工程菌株,共培养时间和恢复时间第一次进行了试验并优化了条件。
- The nobility usually resist social changes. 贵族通常反对社会变革。
- What's the going rate of computer programmers? 计算机程序设计人员的一般工资是多少?
- They are determined to resist invasion. 他们决定抵抗入侵。
- By power we mean the rate of doing work. 我们说功率就是指做功的速率。
- I can never resist an ice cream. 我一见到冰淇淋就忍不住想吃。
- They charge a fixed rate for each transaction. 他们对办理的每项业务收取一定的费用。
- The going rate for freelance work is 5 an hour. 自由职业者的现行酬金是每小时5英镑。
- He could not resist the temptation to steal. 他经不起想要偏巧的诱惑。
- He couldn't resist the lure of money. 他不能抵制金钱的诱惑。
- At that rate, he ought to be punished. 如果那样,他应该受到惩罚。
- This special coating is designed to resist rust. 这一特别的涂层旨在抗锈。
- Can you tell me the rate for a double room, please? 请告诉我一间双人房的费用可以吗?
- He was helpless to resist the temptation. 他无法抵御诱惑。