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Reverse transcription the RNA to cDNA by RT-PCR method. 应用RT-PCR将RNA逆转录为cDNA。
The cDNAs to the BMV-E-RNAs and RNA3a were preparedby reverse transcription. 采用NorthernBlot杂交分析法分别和E株系、G株系的RNA组份进行分子杂交实验;发现RNA3a和RNA3的核苷酸序列有同源性.
Influenza virus sample in cultured cells was treated for reverse transcription, amplification, fluorescent dye labelling, hybridization and scanning. 对待检样品进行随机逆转录和扩增，荧光标记，杂交，扫描分析。
The proto- oncogene c-fos mRNA expression was assessed using reverse transcription polymerase chain reaction(RT-PCR). 用逆转录聚合酶链式反应(RT-PCR)检测心肌细胞原癌基因c-fos mRNA的表达。
TLR 4 mRNA in KCs was determined by the reverse transcription polymerase chain reaction (RT-PCR). 用逆转录多聚酶联反应 (RT -PCR)测定KCs中TLR4mRNA的表达 ;
First , the Oligo dT-3sites Adaptor primer was used to conduct reverse transcription. 首先，以试剂盒提供的Oligo dT-3sites Adaptor primer为引物进行逆转录；
The expression of Igf2 in liver was detected by real-time quantitative reverse transcription (RT-PCR) and Western blot. 用实时定量-逆转录PCR检测孕20天胎鼠肝脏组织Igf2基因mRNA的表达;
Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR). 方法采用逆转录聚合酶链反应（RT－PCR）检测84例静脉毒瘾者血浆标本。
Methods: Mainly with methods of in situ reverse transcription polymerase chain reaction (In situ RT-PCR). 方法以原位逆转录聚合酶链式反应(In situ reverse transcription polymerase chain reaction,In situ RT-PCR)为主要研究方法。
Then the expression of ATF was analyzed by means of reverse transcription polymerase chain reaction (RT-PCR). 用反转录PCR(RT-PCR)检测转染细胞ATF基因的表达;
We amplified SSU rRNA of Leishmania donovani with primer R222 and R333 by reverse transcription polymerase chain reaction. 我们采用引物R222和R333建立杜氏利什曼原虫SSUrRNA逆转录多聚酶链反应（RT－PCR）。
TAUT and CSD mRNA levels were assayed using competitive quantitative reverse transcription polymerase chain reaction (RT-PCR). 竞争性RT-PCR测定TAUT和CSD mRNA的含量.
Reverse Transcription (RT) PCR. The mRNA expressions of cyclin D1, cyclin B1, CDK4, cdc2 and p21 were determined by RT-PCR semi-quantitatively. 逆转录PCR(RT-PCR)：应用RT-PCR半定量检测细胞cyclin D1、cyclin B1、CDK4、CDK1、p21的mRNA表达。
The detecting method of multi-drug resistance gene (MDR-1) expression was established by the reverse transcription polymerase chain reaction (RT-PCR). 采用逆转录?多聚酶链反应双酶单管PT-PCR法，建立了检测肿瘤细胞MDR-1基因表达的方法。
METHODS The reverse transcription polymerase chain reaction(RT PCR) was used to detect expression of mdr 1 mRNA in 20 RCC and 5 normal kidneys. 方法应用逆转录?聚合酶链反应（RT?PCR）技术检测20例肾癌和5例正常肾组织mdr?1基因mRNA的表达。
Using reverse transcription PCR, kininogen mRNA was also detected in lamprey gut, kidney, and leukocyte, but absent in lamprey buccal gland. 运用反转录PCR技术，检测出激肽原还存在于七鳃鳗的肠、肾和白细胞中，但不存在于口腔腺中。
The expression of TNF- a mRNA in the lung tissues was detected by reverse transcription polymerase chain reaction (RT-PCR). 用逆转录-聚合酶链反应(RT-PCR)检测各组肺组织TNF-a mRNA表达;
The expressions of Slit2 were tested by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical measurement. 分别通过逆转录聚合酶链反应法(RT-PCR)和免疫组织化学方法检测Slit2基因的表达水平及部位。
In this section we detected mRNA expression of MMP-9 and its inhibitor TIMP-1 with reverse transcription polymerase chain reaction technique. 采用逆转录PCR技术对MMP-9mRNA及其特异性抑制物TIMP-1mRNA表达水平进行半定量分析。
The genome of NS5A and HVR1 region were amplified by the methods of Reverse Transcription Polymerase Chain Reaction ( RT - PCR). HCV HVR1及NS5A基因片断扩增：采用巢式逆转录聚合酶链反应法(RT-PCR)。