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- Methods Polymerase chain reaction(PCR) connected with reverse dot blot(RDB) were performed. 方法采用多聚酶链反应(PCR)结合反向斑点杂交(RDB)技术。
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
- Keywords Beta-thalassemia;minority;PCR;reverse dot blot（RDB） hybridization; 地中海贫血;少数民族;PCR-反向点杂交;
- We skillfully combinated PCR and reverse dot blot, using thehigh specificity of reverse dot blot overconming the deficiency ofhigh false positive rate of PCR as well as making use of thecharacteristic of high specificity of PCR. PCR-RDB检测方法将PCR技术和反向点杂交方法有机的结合起来，既利用了PCR技术灵敏度高的优点，又利用反向点杂交技术的高、度特异性克服了PCR方法假阳性率高的缺点，在实验室和现场的初步应用中都取得了较好的结果。
- Keywords N-acetyltransferase （NAT2）;polymorphism;reverse dot blot （RDB）;allele specific oligoneucliotides （ASO）;genotyping; 乙酰化酶（NAT2）;多态性;反向点杂交法（RDB）;等位基因特异性寡核苷酸探针（ASO）;基因分型;
- PCR-flow through reverse dot blot PCR-流过式反向斑点杂交
- reverse dot blot (RDB) 反向点杂交法
- 10 Zhang JZ,Xu XM.Ma WF,et a1.A rapid reverse dot blot assay [张基增.;徐湘民
- SCREENING MUTATIONS IN PHENYLKETONURIA BY MEANS OF NONRADIOACTIVE REVERSE DOT BLOT HYBRIDIZATION 非同位素逆相点杂交方法检测苯丙酮尿症突变基因
- Detection and genotyping of Chlamydia trachomatis by a reverse dot blot procedure in urogenital specimens 反向斑点杂交技术检测泌尿生殖道沙眼衣原体及基因分型
- Rapid detection of mycobacterium tuberculosis isoniazid-resistant genes by membrane reverse dot blot hybridization 应用膜反向斑点杂交技术检测耐异烟肼结核分支杆菌基因型
- Rapid diagnosis the genotype of Ureaplasma urealyticum by Reverse dot blot and clinical significance 反相斑点杂交快速诊断解脲脲原体基因型及其临床意义
- Rapid identification of mycobacterium species by amplification of rpoB gene and reverse dot blot hybridization assay 应用rpoB基因PCR反向斑点杂交快速鉴定分枝杆菌菌种的研究
- Keywords thalassemia intermedia;polymerase chain reaction;reverse dot blot hybridization;prenatal diagnosis; 中间型地中海贫血;聚合酶链反应;反向斑点杂交;产前诊断;
- Keywords Thalassemia;Gene;Heterozygote;Polymerase chain reaction;Reverse dot blot; 地中海贫血;基因;杂合子;聚合酶链反应;反向点杂交;
- Detection of drug-resistances of Mycobacterium tuberculosis isolates to streptomycin and ethambutol by a new method of reverse dot blot 用反向斑点杂交法检测结核杆菌对链霉素和乙胺丁醇的耐药性
- Rapid Typing of HPV by Reverse Dot Blot Hybridization and Its Clinical Characteristics Among Patients with Condyloma Acuminata 尖锐湿疣HPV导流杂交快速基因分型及临床意义
- Detection of rpoB mutations in rifampin resistant Mycobacterium tuberculosis strains by the reverse dot blot hybridization method 应用反向斑点杂交法检测耐利福平结核分支杆菌rpoB基因突变
- Keywords Polymerase chain reaction;Reversed dot blot;Ureaplasma urealyticum; 聚合酶链反应;反相斑点杂交;解脲脲原体;
- Effect of labeling was detected by dot blot hybridization. 点杂交方法检测探针标记效果。
