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- Methods Polymerase chain reaction(PCR) connected with reverse dot blot(RDB) were performed. 方法采用多聚酶链反应(PCR)结合反向斑点杂交(RDB)技术。
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
- Keywords Polymerase chain reaction;Reversed dot blot;Ureaplasma urealyticum; 聚合酶链反应;反相斑点杂交;解脲脲原体;
- We skillfully combinated PCR and reverse dot blot, using thehigh specificity of reverse dot blot overconming the deficiency ofhigh false positive rate of PCR as well as making use of thecharacteristic of high specificity of PCR. PCR-RDB检测方法将PCR技术和反向点杂交方法有机的结合起来,既利用了PCR技术灵敏度高的优点,又利用反向点杂交技术的高、度特异性克服了PCR方法假阳性率高的缺点,在实验室和现场的初步应用中都取得了较好的结果。
- The train arrived on the dot of three o'clock. 那辆火车三点钟准时到达。
- He said he used this system in the year dot. 他说他很久以前用过这个系统。
- He went away, limping dot and go one. 他一拐一拐地跛着走开了。
- He's very punctual always arrives on the dot. 他很守时--总是按时到。
- He reversed the coin and examined it carefully. 他把硬币倒过来,仔细检查。
- Husband and wife have reversed roles. 丈夫和妻子互换了责任。
- The court reversed its decision. 法庭撤销了原来的判决。
- He reversed his car into the garage. 他把车子倒开进车库。
- She was told to arrive at 8 o'clock on the dot. 她被告知八点钟准时到。
- His coat can be reversed when it begins to rain. 他的外衣下雨时能翻过来穿。
- Writing is reversed in a mirror. 写出来的字在镜子里是倒著的。
- PCR-flow through reverse dot blot PCR-流过式反向斑点杂交
- Their situations are now reversed as employee has become employer. 他们双方的地位转换了,雇员成了雇主。
- Something that has been reversed; an opposite. 相反事物被颠倒的事物; 对立物
- The committee reversed its decision on import quota. 委员会取消了对进口货物的配额的决定。
- He watched the train away until it was only a dot in the distance. 他目送着列车驶去,直到它成了远方的一个小黑点。