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To set up a sephadex column chromatography method for the determination of polymer impurity in cefotetan disodium. 目的建立葡聚糖凝胶色谱法测定头孢替坦二钠中聚合物的方法。
Keywords sephadex column chromatography;cefotetan disodium;polymer; 葡聚糖凝胶色谱;头孢替坦二钠;聚合物;
Keywords polysaccharides of Spirulina;platensis;sephadex column chromatography;elution; 螺旋藻多糖;凝胶柱层析;洗脱;
sephadex column chromatography 交联葡聚糖柱色谱法
A protease purified from the fresh leaves of Acer truncatum Bunge by means of Sephadex A-50, Sephadex G-200 column chromatography. 采用Sephadex A-50;Sephadex G-200柱层析的方法;从槭树科槭树属元宝枫(AcertruncatumBunge)的叶片中提取并纯化了叶蛋白酶.
Twoelution peaks were obtained on the Sephadex G100 column chromatography of the crude extractand the active substance was found in the first peak. 粗提液经 Sephadex G100层析后,得到了两个洗脱峰,活性物质存在于第一峰中。
The fibrinolytic principle was obtained by twice DEAE-Sephadex A-50 column chromatography and Sephadex G-100 gel filtration from the venom of Agkistrodon halys Pallas. 用二乙氨基乙基葡聚糖A-50两次柱层析及葡聚糖凝胶过滤,从浙江产蝮蛇毒中得到一个纤溶组份。
It was efficient to use Sephadex G 50 column chromatography and ultra violet absorption spectrometry atomic absorption spectrophotometry in isolation, and then it was followed by quantitative analysis of chromium binding substances. 采用葡聚糖凝胶G?50柱层析法分离低分子蛋白效果好。
A total of 20 compounds were isolated from the strain F121112 by the column chromatography on silica gel, RP-Cig and Sephadex LH-20, followed by reverse-phase high performance liquid chromatography. 采用生物活性跟踪分离的方法,通过硅胶柱层析、ODS C-18柱层析、Sephadex LH-20凝胶层析以及RP-HPLC等各种色谱技术,对F121112产生的生物活性物质进行分离纯化,得到了20个化合物。
After a long process of experiment, finally isolate the active fraction from Flos Daturae for psoriasis with Sephadex LH-20 column chromatography and water-methol solvent system successly. 本文经过长期反复的摸索,最终采用葡聚糖凝胶(Sephadex LH-20)柱色谱以水-甲醇溶剂系统成功地对洋金花治疗银屑病的有效部位进行了分离。
Methods The compounds were isolated by column chromatography with silica gel and purified by Sephadex LH-20 and ODS-HPLC. The structures were identified by means of NMR and MS analyses(1H-NMR,13C-NMR,and ESI-MS). 方法利用正相硅胶柱色谱、葡聚糖凝胶Sephadex LH-20、反相制备色谱等手段进行分离纯化,并通过1H-NMR、13C-NMR、ESI-MS等波谱技术进行结构鉴定。
The molecular mass of the PLC was determined as 64 kDa using SDS-PAGE, which was in agreement with the results obtained from Sephadex G-200 column chromatography (65 kDa), suggesting that the PLC was a monomeric protein. SDS-PAGE电泳显示该磷脂酶C呈现单一条带,分子量约为64 kDa。通过Sephadex G-200凝胶过滤层析柱标定的磷脂酶C的分子量为65 kDa,两者的结果一致,表明该酶是单体蛋白质。
Galangin was obtained by on-step column chromatography of polyamide only once. 运用聚酰胺柱层析－次分离，获得有效成分高良姜素。
Its homogeneity was identified with Sepharose 6B column chromatography. 紫外光谱显示它不含蛋白质、多肽及核酸。
In this paper, we extracted the antioxidative substance from Alpinia oxyphylla Miquel by using column chromatography. 摘要探讨了干柱层析法分离益智渣及益智乙醇提取物的抗氧化成分。
METHODS: Alcoholic extraction, silica gel, ODS column chromatography and recrystallization were used. 方法:采用工业酒精提取、胶柱层析、DS柱层析和重结晶等方法。
METHODS CTX-d was fractionated and purified from Naja naja atra venom by column chromatography and RP-HPLC. 方法应用柱层析及RP-HPLC,从眼镜蛇毒粗毒中分离纯化细胞素素(CTX)。
Results Galangin was obtained by on-step column chromatography of polyamide only once. 结果运用聚酰胺柱层析-次分离，获得有效成分高良姜素。
Objective To separate flavonol and flavanonol by the column chromatography of polyamide. 目的采用聚酰胺柱色谱分离黄酮醇与二氢黄酮醇类化合物。
Results Pure flavonol and flavanonol were obtained by the column chromatography of polyamide. 结果运用聚酰胺柱色谱法一次分离得高纯度黄酮醇与二氢黄酮醇。