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- sequence specific probe 顺序特异性引物
- Methods HLA-A and B alleles polymorphism in 65 patients with leukemia and 48 normal subjects were determined by PCR with sequence specific oligucleotide probe (PCR-SSO). 方法采用序列特异性寡核苷酸探针杂交技术(PCR-SSO)对65例白血病患者和48例正常对照组健康者进行HLA-A、B基因分型。
- 12 cases(44%) aberrations were detected by two sequence specific probes for D13S272(13q14.3),ATM(11q23),9 with del(13q14.3),7 with del(11q23). 两种位点特异性探针检出12例(44%25)异常;del(13q14.;3)9例;del(11q23)7例。
- Methods HLA-A, B alleles polymorphism in 197 cases with ALL and 5841 normal umbilical cord blood controls were determined by PCR with sequence specific olignucleotide probe(PCR-SSO). 方法采用聚合酶链反应-序列特异性寡核苷酸探针(PCR-SSO)方法,对197例北方汉族儿童ALL患者和5841例健康脐带血样本HLA-A,B等位基因多态性进行研究。
- A cDNA clone of 1 629 bp was obtained by screening the library with an adult stage specific probe. 利用消减杂交 (subtractedhybridization)获得的旋毛虫成虫期特异性探针筛选成虫cDNA文库 ,获得一个长 16 2 9bp的基因。
- Methods:We used telomerase PCR MPH,an assay in which a digoxigenin labeled telomerase repeat specific probe was hybridized to the PCR product. 方法:PCR-MPH以地高辛标记端粒重复片段特异的探针与PCR变性产物杂交,经酶底物显色,通过测定吸光度判断结果;
- Peptide nucleic acids(PNA) are DNA mimics that bind strongly to DNA and RNA in a sequence specific manner. 肽核酸(PNA)是DNA模拟物,能与DNA和RNA以序列特异性方式结合,在基因研究和基因治疗方面有广泛的应用前景。
- Peptide nucleic acids (PNA) are DNA mimics that bind strongly to DNA and RNA in a sequence specific manner. 肽核酸(PNA)是DNA模拟物,能与DNA和RNA以序列特异性方式结合,在基因研究和基因治疗方面有广泛的应用前景。
- Not only are ASON able to inhibit HBV gene expression with sequence specific but also clear HBV in the way of apoptosis. ASON不仅以序列特异性方式发挥抗病毒作用 ,而且可能以凋亡的方式清除HBV
- Chemical nucleases are newly designed and synthesized compounds which used as tools of DNA or RNA cleavage in a sequence specific way. 化学核酸酶是一类人工设计、合成的DNA 或RNA 定位断裂工具, 由核酸识别结合系统和化学断裂系统组成。
- The DNA electrochemical sensor was prepared by covaltently binding the designed CML DNA specific probe on the surface of GCE electrode together with MB as electro-active indicator for the first time. 本文利用共价结合法,首次将人工合成的慢粒基因片段做为特异性探针固定在玻碳电极表面,结合电活性指示剂MB构建DNA电化学传感器。
- Abstract: Chemical nucleases are newly designed and synthesized compounds which used as tools of DNA or RNA cleavage in a sequence specific way. 摘要: 化学核酸酶是一类人工设计、合成的DNA 或RNA 定位断裂工具, 由核酸识别结合系统和化学断裂系统组成。
- The sequence specific primers of the gene mutation of the mannose combining agglutinator gene exon I at 52 site condon was detected with polymerase chain reaction. 进行甘露糖结合凝集素外显子I52位密码子基因突变的序列特异性引物聚合酶链式反应检测。
- sequence specific oligonucleotide probe hybridization SSOPH
- sequence specific oligonucleotide probe hybridization(SSOPH) 序列特异寡核苷酸探针杂交
- Flow-reverse sequence specific oligonucleotide probe 特异性寡核苷酸探针
- RFLP markers were used to analyze 58 rice materials by 45 indica-japonica specific probes. 用45个籼粳特异探针,对供试亲本材料进行RFLP分析;
- The results showed that caspase-3 ASODN inhibited apoptosis with sequence specificity and concentration relativity. 结果显示,Caspase-3 mRNA ASODN抑制细胞凋亡具有序列特异性和剂量相关性。
- Methods DNA samples from 183 random healthy individuals in Han population, 41 in Dai population and 66 in Uygur population were genotyped by using the polymerase chain reaction sequence specific oligonucleotide probing (PCR SSOP) method. 方法 采用聚合酶链反应 -序列特异的寡核苷酸探针杂交 (polymerase chain reaction andsequence- specific oligonucleotide probing,PCR- SSOP)方法 ,分析 183名汉族、4 1名傣族和 6 6名维吾尔族正常人群的 MICA胞外区等位基因多态性。
- One species-specific probe of Yersinia pestis is successfully screened by PCR fingerprinting, and the method presented in this paper proves to be a novel way to get specific probes of pathogenic bacteria. 利用PCR指纹图技术成功筛选到一段鼠疫耶尔森菌的种特异性探针 ,为细菌通用检测技术的研究开辟了新的思路。