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- Keywords stem cells;immunohistochemistry;culture media;serum free;nervous system/cytology;nervous system/embryology;cell culture; 干细胞;免疫组织化学;培养基;无血清;神经系统/细胞学;神经系统/胚胎学;细胞培养;
- AIM To observe the effects of epidermal growth factor (EGF) and bovine pituitary extract (BPE) on human pituitary adenoma cells cultured in serum free medium. 目的 观察表皮生长因子 (EGF)和牛垂体提取物(BPE)在人垂体腺瘤细胞培养中的作用 .
- Methods:Under serum free culture media, MSCs were incubated with different doses of EP for 24 h(0, 0.25, 0.5, 1, 2, 10 U/ml), and then the proliferation of MSCs was measured by MTT assay. 方法:无血清条件下;不同剂量(0;0.;25;0
- And results from TUNEL and trypan staining showed that both EGF and BPE of different concentrations decreased the number of apoptosis and death of human pituitary tumors cells cultured with serum free medium in vitro . 同时 ;EGF和BPE均可降低体外无血清培养的人垂体腺瘤细胞的死亡及凋亡数量 .
- GF and BPE have an important role in human pituitary tumors cells cultured in serum free medium in vitro . EGF and BPE can promote the proliferation of human pituitary tumors cells cultured in vitro . EGF和BPE具有明显改善人垂体腺瘤细胞体外无血清培养条件的作用 ,EGF和BPE对离体培养的垂体腺瘤细胞增殖具有促进作用
- After incubation with Cpd 861 for 48 hours, HSC was harvested to determine collagen type I,III,IV mRNA by dot blot hybridization and cell culture medium was used to detect collagen secretion by ELISA method. 传一代细胞以10mg/ml复方861作用48小时后 ,以ELISA法、斑点杂交法检测胶原蛋白分泌及相应mRNA水平的变化。
- A SV_(40) late replacement expression vector containing secreted neuraminidase cDNA was transfected into COS cells. High nenraminidase activity could be detected in the cell culture medium. 将含分泌型甲型流感病毒神经氨酸酶cDNA的SV_(40)晚期区段取代载体转染COS细胞后,可在细胞培养液中测出高酶活力。
- Methods LoVo cell line was cultured using nitrocellulose membrane as the culture medium, in parallel with the same cell culture on the coverglass which served as control. 方法应用硝酸纤维素膜培养细胞和病理染色并与常规方法培养细胞的比较。
- Methods Expression vector pCTLA 4/Ig was transfected into COS 7 with lipid reagent. The expression of fusion protein in the supernatant of the cell culture media was detected with double antibody sandwich ELISA. 方法 将pCTLA 4/Ig表达质粒转染COS 7细胞 ,双抗体夹心ELISA检测细胞培养上清中融合蛋白表达 ;
- Objectives:To study the osteoblastic differentiation ability of rabbit bone marrow stromal cells cultured in experimental culture medium. 目的:观察条件培养液对兔骨髓基质细胞向成骨细胞分化的影响。
- Among the SK-PLAR clones studied, SK-PLAR54 can differentiate neuron-like morphology upon 3 days culture in serum free medium. 然而，有报导基因表现的细胞株，其报导基因表现不受雄性素影响，呈现持续性表现。
- Method: The stromal cells were cultured in proliferative stage after the treatment of hormones then the stromal cells culture medium (SCM) was collected. 方法：将增生期子宫内膜间质细胞经激素处理后进行培养，提取培养液。
- Free Cell solitaire is a very famous game. 免费细胞纸牌是一个非常著名的游戏。
- In addition, some culture media supernatant of directly irradiated cells were transferred to the non-irradiated cells culture medium, forming the group P2. 另将直接受照细胞培养液转移至未受照细胞培养瓶中形成旁效应2组。
- Primary study of tracheal membrane epithelium cells cultured by serum free medium 气管黏膜上皮细胞无血清培养的初步实验研究
- Abstract： Objective To observe the effect of supernant of pulmonary microvascular endothelial cell cultured medium (SPMECCM) on rat aortic smooth muscle cells (ASMC) in hypoxia condition. 提 要 ： 目的 观察缺氧条件下培养的肺微血管内皮细胞（PMEC）培养液对大鼠主动脉平滑肌细胞（ASMC）的作用。
- Serum free amino acid was measured before and after the treatment. 结果：经碳酸盐透析后，患者血浆大多数游离氨基酸和总氨基酸含量显著降低。
- Objective To establish an adult bovine lens epithelial cells culture system, and investigate the growth characteristics of the cells in different culture media and its ultrastructure. 目的建立成年牛眼晶状体上皮细胞的培养方法,研究其在不同培养液中的生长特性并观察其超微结构。
- The Sika Deer splenocytes culture supernatant has action on the proliferation of NIH3T3 cells,and also can promote the wound healing of NIH3T3 cells. In the serum free medium this culture supernatant can also promote the proliferation of NIH3T3 cells. 梅花鹿脾细胞培养上清对正常小鼠 NIH3T3细胞具有促增殖作用 ,对创伤的 NIH3T3细胞具有促进愈合作用 ,并且在无血清培养液中此上清对正常 NIH3T3细胞也具有促增殖作用。
- The games are: Klondike, Free Cell, Touch 21, Touch Towers and Win11. 游戏是：Klondike，自由的细胞，触摸 21，按塔和 Win11。
