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- Culture temperature 24-28 ?, light time 16h, explant light side towards up, the proporationable hormone concentration with MS+2.0mg/lBA+4.5mg/lAgNO3 were the optimum conditions, under which shoot regeneration frequency could reach 92%. BA是西瓜子叶再分化必需的物质,AgNO_3有效提高芽分化率,两激素浓度的最佳配比为MS+2.;0mg/lBA+4
- The green shoot regeneration frequencies were 0.37% with PPA-based one-step medium, 0.21% with M8 medium and 0.14% with SH medium. It indicated that the PAA based one-step medium was most effective in the anther culture of indica genotypes. 其中,一次成苗培养基、M_8培养基和SH培养基花培绿苗率分别为0.;37%25,0
- Shoots regeneration frequency increased significantly when cotyledons were preconditioned in MSB5 medium with 1 mg/L 2,4-D and 1 mg/L BA for 3 days and then cultured in MMO medium containing 4.5 mg/L BA. 将子叶在附加1 mg/L BA和1 mg/L 2,4-D的MSB5培养基中预培养3天,再转入含有4.;5 mg/L BA的MMO培养基中培养,可显著增加芽再生率,并且可将再生周期比对照缩短1/3。
- shoot regeneration frequency 植株再生率
- A shoot regeneration system from cotyledon of Helianthus annuus L. 油葵子叶外植体不定芽再生体系的建立。
- The frequency of shoot regeneration from cotyledon explants of fifty days fast vegetable and Zhejiangzaoshu No. 5 is higher. So both varieties were used as the material for transgenic experiment. 子叶外植体的芽分化率以50天快菜和浙江早熟5号为高,故此以这两个品种作为转基因的试验材料。
- An Efficient Method for Shoot Regeneration from Cotylendons of Cucu rmis melo L. 甜瓜再生芽高效诱导方法的研究。
- Adventitious Shoot Regeneration from in vitro Cultured Leaves of Platanus orientalis Willd. 悬铃木叶片不定芽再生。
- Morphological and physiological maturities of somatic cells directly affect plantlet germination and plantlet regeneration frequency. 体细胞胚在形态和生理上的成熟,直接影响到植株的萌发和再生频率。
- Effects of Nursing Culture and Section of Cotyledon on Shoot Regeneration of Brassica juncea Coss. Gemmifera Lee et Lin in vitro Culture (看护培养和子叶部位对儿菜(Brassica juncea Coss.
- Studies on the enhancement of plant regeneration frequency in Brassica napus in vitro culture. 提高甘蓝型油菜离体培养再生频率的研究。
- Osmotic stress promotes shoot regeneration in immature embryo-derived callus in rice (Oryza sativa L.). 渗透压诱导水稻愈合组织植株再生过程中过氧化酵素之变化。
- A speedy and high efficient procedure for shoot regeneration from cotyledons of winter and semi-winter type Brassica napus L. 甘蓝型冬性和半冬性油菜子叶高效快速芽再生体系的建立。
- In the experiments, the optimal selection pressures of kanamycin for shoot regeneration and rooting were also determined. 在此基础上,确定了不定芽再生和生根的卡那霉素选择压。
- Xu XH, Davey MR : Shoot regeneration from mesophyll protoplasts and leaf explents of Rehmannia glutinosa. Plant Cell Rep. 1983; 2:55-57. 张东迪:地黄组织培养之研究,中国医药学院中国药学研究所硕士论文,台中1988。
- The results showed that the cotyledon cultured for 3 days has the highest regeneration frequency of 95.5%,when it was placed in the MS basic medium supplemented with BA0.4mg/L. Furthermore,adventitious bud was appeared after 12 days culture. 结果表明:以MS为基本培养基;附加BA0.;4mg/L处理时;3d苗龄的子叶的分化频率最高;可达95
- However, too hypo regeneration frequency restricted transgene project developing of grape now. Conquering this conundrum and improving regeneration became the hot spot research in grape biotechnology breeding. 但是葡萄转基因工程目前发展的瓶颈是葡萄再生频率低,突破再生频率这一难题,使其高效地再生是葡萄生物技术育种在当前的研究热点。
- Rose bushes shoot again after being cut back. 玫瑰丛修剪後还能再长出新枝。
- The results showed that when the concentration of Ampicillin was at 500 mg/L, the rates of callus induction and shoot regeneration from the leaves of Bartlett were 96.48% and 56.17% respectively, which were not significantly different from that of CK. 结果表明;Amp对巴梨叶片分化影响较小;当浓度达500mg/L时;其出愈率和不定梢再生率分别为96.;48%25和56
- Optimal concentrations of 6-BA and NAA and their combinations were tested with quadratic regressive orthogonal design for their effect on shoot regeneration of Dianthus caryophyllus L. 以香石竹叶片为外植体,利用二次回归正交设计对其再生体系建立中的关键因素6-BA和NAA的浓度及其组合进行定量优化。