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shuttle vector of adenovirus 重组腺病毒穿梭载体
The Pseudorabies virus (PRV) Fa strain gC gene was subcloned into Adenovirus shuttle vector pDC316 through double enzymatic digestion. 通过双酶切将伪狂犬病毒(Pseudorabies virus,PRV)Fa株gC囊膜糖蛋白基因片段亚克隆到5型腺病毒AdMax系统穿梭质粒pDC316中,得到pDC316-gC。
Construction of an inducible and efficient expression-secretion shuttle vector of B. Subtilis 双功能枯草杆菌诱导型高效表达分泌载体的构建与鉴定
Construction of shuttle vector of anthrax toxin protective antigen with upstream strong promoter 芽孢杆菌外毒素保护性抗原与上游强启动子穿梭载体的构建
Each tuple is a vector of members. 每个元组是一个成员向量。
The radius vector of each solid is always a positive length. 每个立体的矢径总是正值长度。
The Tat signal peptide detecting vector of PGA were constructed. 构建了Tat信号肽检测载体。
We also have constructed two shuttle vector plw9,psc11m1 which can recombine to FPV genome. 我们还构建了可以和FPV基因组发生重组的穿梭载体，plw9和psc11。
Objective To test the anti-tumor effect of adenovirus vector generated from the homologous recombination of bacteria mediating TK gene on hepatic neoplasm cell. 目的探讨用细菌内同源重组法制备含TK自杀基因腺病毒对肝癌细胞的杀伤作用。
The recombinant shuttle vector pBacPAK-CTB and pBacPAK-4aa-CTB was co-infected with Bm-BacPAK6 DNA into BmN cells. The recombinant virus was screened, plaque-purified and identified by PCR and Southern hybridization. 重组转移载体DNA与经Bsu36I酶切线性化的修饰型病毒Bm-BacPAK6 DNA共转染家蚕BmN细胞，然后经空斑纯化、PCR扩增、Southern杂交等方法鉴定出含CTB基因的重组病毒rBm-BacCTB和rBm-Bac4aa-CTB。
Fleas are vectors of the plague. 跳蚤是瘟疫的传播媒介。
As the pMCLacI/neo shuttle vector is a novel bi-functional mutation detection system, apart from be used in above transgenic animal study , it was also used in our study of MNNG-indued DNA damage and repair research. 由于本研究率先在突变检测系统中应用了具有双功能的pMCLacI/neo穿梭载体,在利用转基因动物技术建立突变检测系统及展开相关研究的同时，我们又利用基因敲除技术，定向敲除DNA损伤修复酶基因-MGMT，建立了一系列MGMT基因定向敲除的突变检测系统，及高表达MGMT的突变检测系统，并展开了相应的DNA损伤修复的研究。
The target fragments were cut down from pGEMT-easy and ligated with shuttle vector pJFF224-XN/E. coRI. Through identification, the homogenous regulatory gene inserted in ?cis? and ?trans? direction under T4 promoter, separetely. 切下目的片段与酶切穿梭质粒pJFF224-XN连接,经PCR及酶切鉴定,调节基因片段分别以正向或反向插入到质粒的T4启动子下。
The target fragments were cut down from pGEMT-easy and ligated with shuttle vector pJFF224-XN/E. coRI. Through identification, the homogenous regulatory gene inserted in cis and trans direction under T4 promoter, separately. 切下目的片段与酶切的穿梭质粒pJFF224-XN连接,经PCR方法及酶切鉴定,调节基因片段分别以正向或反向插入到质粒的T4启动子下;
Then, the DNA fragment xylR-gfp which were obtained after pGEM-xylRGFP being digested by Kpn I and Sph I , was inserted into Kcoli-B.subtilis shuttle vector pRP22, and the resulted recombinant plasmid was named at pRP22-GFP. 经SphI/KpnI双切质粒pGEM-xylRGFP后，回收片段xylR-gfp，插入穿梭载体pRP22，得到重组子pRP22-GFP。
The vector Z is known as the state vector of the system. 向量Z称为系统的状态向量。
With the development of adenovirus vector, the adenovirus will play a more and more important role in controlling human and animal diseases, especially viral diseases as well as in human gene therapy. 随着动物腺病毒载体的发展，它将在控制人和动物传染性疾病，特别是病毒性传染病以及人类的基因治疗中发挥越来越重要的作用。
The strain BSG is more efficient recipient of transformation for shuttle vectors pPTE4 and pBE2 from E. 来自大肠杆菌的穿梭质粒在BSG菌中转化频率较亲本高。
E. coli-B. subtilis shuttle vector 穿梭载体
Conclusion:The infection efficiency of adenovirus is determined by the levels of CAR and integrin on cancer cells. 结论：肿瘤细胞表面CAR和整合素的表达水平决定了腺病毒对肿瘤细胞的感染效率。