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single cell gel electroporesis 单细胞凝胶电泳
Single cell gel electrophoresis(SCGE), which has founded in 1980s, can detect the DNA injury in single cell. 单细胞凝胶电泳技术建立于20世纪80年代,用于检测单个细胞的DNA损伤。
The ability of trichloroethylene(TCE) to induce single strand breaks in peripheral lymphocytes DNA of male mice in vivo was evaluated using single cell gel electrophoresis(SCGE). 为研究三氯乙烯（TCE）的遗传毒性，应用单细胞凝胶电泳法（SCGE）测定了TCE诱导人和小鼠外周血有核细胞DNA链断裂的能力。
The anti-mutagenicity of reduced coenzyme NADH was studied by Ames assay and single cell gel electrophoresis assay (SCGE, comet assay). 采用Ames实验及单细胞凝胶电泳(SCGE,彗星实验),对还原型辅酶NADH进行抗突变研究。
The single cell gel electrophoresis(SCGE) also called commet assay is a method which can detect DNA rupture under level of single cell. 单细胞凝胶电泳(SCGE)又称为彗星实验,是一种可以在单个细胞水平上检测DNA断裂的技术。
Purpose and Methods: Single cell gel electrophoresis(SCGE), sister chromatid exchanges(SCE), chromosome aberrations are three methods to determine DNA damage and repair. 目的与方法 :单细胞凝胶电泳技术 (SCGE)、姐妹染色单体交换法 (SCE)和染色体畸变分析法均能被用来检测DNA的损伤或修复。
Objective: Traditional Ames test, in vitro mammalian chromosome aberration test, single cell gel electrophoresis assay have been used widely in monitoring chemical toxicity. 目的:传统的Ames实验、体外哺乳动物细胞染色体畸变实验、单细胞凝胶电泳实验已广泛用于化学品毒性的监测,但用于综合评价化学品的遗传学毒性还不够完善。
Single cell gel electrophoresis is applied for genetic toxicology,DNA damage and detection,environmental pollution detection,apoptosis mechanism research et al. 单细胞凝胶电泳被广泛应用于遗传毒理学、DNA损伤与修复的检测、环境污染检测、细胞凋亡机制的研究等诸多领域。
The sertoli/germ cell culture and single cell gel electrophoresis (SCGE) was used to detect the DNA damage of spermary cell in mice, which was induced by tested m-nitrobenzene. 采用小鼠睾丸支持细胞/生殖细胞共培养法以及单细胞凝胶电泳技术,研究了间二硝基苯对小鼠睾丸生殖细胞DNA的损伤作用。
Methods Semen samples from soldiers with different job categories were analyzed by single cell gel electrophoresis(SCGE)and computer assisted sperm analysis(CASA). 方法采用单细胞凝胶电泳和精子全自动分析相结合的方法对两组不同职业男性的精液标本进行检测。
Single cell gel electrophoresis is applied for genetic toxicology, DNA damage and detection, environmental pollution detection, apoptosis mechanism research et at. 单细胞凝胶电泳被广泛应用于遗传毒理学、DNA损伤与修复的检测、环境污染检测、细胞凋亡机制的研究等诸多领域。
The single cell gel electrophoresis(SCGE),also called comet assay,is a sensitive and rapid method for DNA damage detection in individual mammalian cell. 单细胞凝胶电泳是一种在单细胞水平检测哺乳类有核细胞DNA断裂的新技术。
The tail extent, tail inertia, percentage of tail DNA and Olive tail moment are selected as parameters, which could be measured by the fluorescent pictures of single cell gel electrophoresis (SCGE) and IMI 1.0 comet assay analysis software. 观察指标为彗星状荧光图像的彗星尾长、尾%25DNA、尾惯量、Olive尾矩。
Methods DNA damage was tested in HepG2 cells exposed to organic extracts from CDW by using single cell gel electrophoresis assay (SCGE). 方法氯化饮水有机提取物染毒 Hep G2后 ,用单细胞凝胶电泳 (亦称彗星试验 )检测 DNA损伤。
DNA damage induced by radon in bone marrow of mice was detected by m ethods o f single cell gel electrophoresis (SCGE), micronucleus(MN) and laser scanning co nfocal microscope (LSCM) observation. 采用单细胞凝胶电泳（SCGE）、微核（MN）实验、激光共聚焦显微镜（LSCM）检测小鼠骨髓细胞的DNA链断裂、微核细胞率及凋亡率，观察氡暴露致小鼠骨髓细胞的DNA损伤。
Single cell gel electrophoresis(SCGE) is a technique to detect DNA damage of single cell quickly,and is generally applied for reproductive cells. 单细胞凝胶电泳技术(SCGE)是一种快速检测单个细胞DNA损伤的实验技术,在生殖细胞DNA损伤的检测中广泛应用。
Methods The alkaline single cell gel electrophoresis (SCGE) was used to detect the DNA damage of peripheral lymphocytes of woman servants worked in decorated areas or not for half year. 方法应用单细胞凝胶电泳法分别检测在装修场所与未装修场所工作半年女服务员外周血淋巴细胞DNA损伤。
Abstract Purpose and Methods : Single cell gel elect rophoresis ( SCGE) , sister chromatid exchanges ( SCE) ,chromosome aberrations are three methods to determine DNA damage and repair. 摘要目的与方法:单细胞凝胶电泳技术(SCGE) 、姐妹染色单体交换法(SCE) 和染色体畸变分析法均能被用来检测DNA 的损伤或修复。
The single cell gel electrophoresis technique (SCGE) was used to detecte the DNA damage of spermary cell in mice, which were induced by 9 tested environmental endocrine disrupting chemicals. 应用单细胞凝胶电泳技术,研究了烷基酚类化合物对离体小鼠睾丸细胞DNA的损伤作用。