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- In the past few years, many new protocols for directed evolution were developed on the basis of oligonucleotide -directed random mutation, error-prone PCR and DNA shuffling. 最近几年,在过去常用的寡核苷酸介导的随机突变、易错PCR和DNA改组等方法的基础上又出现了一些新的定向进化方法。
- The dot's children varied in length depending on what value the random mutation assigned. 这个点的孩子们的长度随着随机变异赋以参数值而有所不同。
- These anomalies could arise if random mutation reversed the disablement that originally produced the pseudogene. 这种变异型的产生,可能是因为原本让基因失去功能、变成伪基因的缺损,因随机突变而修复了。
- Variation thus became mutation and "mutation" became inseparably compounded into "random mutation. 于是,变异(variation)就变成了突变(mutation),而“突变”又跟随机组合成不可分割的“随机突变”。
- Still more frightening, they would by either design or random mutation develop the ability to communicate with one another. 更可怕的是,它们可能经由计画或随机突变而发展出相互沟通的能力。
- In high organism, the length of intron is much longer than that of extron, and most of random mutation happened in intron. 在高等生物中,内含子的长度远大于外显子,大部分随机突变会发生在内含子中。
- Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various hydroxynitrile lyases. 研究人员已经利用蛋白质工程和定点突变技术来改变各种醇腈酶的活性位点和底物特异性等。
- Any random mutation in an intron that proved beneficial to the host organism would have been retained by natural selection. 插入子内任何偶然的突变,只要对宿主有利,就会在天择的筛选下保留。
- Methods Variation of P gene of HBV polymerase was determined by short segment PCR ELISA according to the recombined plasmid which was acquired by the site directed mutagensis. 方法 通过定点诱变获得的重组质粒作为标准对照 ,采用短片段PCR ELISA方法对拉米呋啶治疗的 6 0例乙肝患者血清标本进行HBV聚合酶P基因的变异检测 ,同时观察其HBVDNA定量、肝功能指标的变化。
- The combining roulette wheel with optimization selection is used.A position-based and group permutation crossover method and random mutation are introduced. 采用轮盘赌与最优性相结合的方法进行选择,设计基于位置成组移位的杂交算子和随机交换的变异算子。
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- The ID movement neither completely denied Darwinism,nor denied the existence of evolution,however,it mostly criticized Darwin and his adherents regard natural selection and random mutation as the uppermost and only root of evolution. ID运动没有全盘的否定达尔文主义,也不否定进化现象的存在,而是批评达尔文及其追随者把自然选择和随机突变作为进化现象最主要的、甚至唯一的根源。
- In order to achieve the achromatic effect of micro-OVD, a directional random speckle structure template base on the low frequency template was designed. 在低频光栅模板的基础上,给出了定向散斑结构输入模板的设计方法,它可进一步改进图像的非彩色效果。
- nucleotide site directed muta-genesis 核苷酸定点诱变
- Random mutations in the DNA sequence which act as genetic milestones or genetic markers. 任意变化在DNA序列作为基因里程碑或基因标志。
- In this paper, a numerical model of wave set ?up and nearshore current generated by directional random wave on complicated mild slop is presented and is tested on the supposed topography . 本文建立了一套计算近岸复杂地形上由二维随机波浪引起的的波浪增水与近岸流的数值模式,并在模拟地形上实施了计算实验。
- The effect of point mutation of KatG gene on the catalase activity associated with INH resistance by site directed mutagenesis 定向诱变方法研究结核分支杆菌KatG基因突变与异烟肼耐药机制的关系(英文)
- Keywords Bim;Grb10;SH2 domain;BH3 domain;yeast two hybrid;sub-cellular location;co-immunoprecipitation;site directed mutation;apoptosis; SH2结构域;BH3结构域;酵母双杂交;细胞定位;免疫共沉淀;定点突变;细胞凋亡;
- Studies on the Two Methods of Gene Random Mutation in vitro 基因体外随机突变的两种方法的研究