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- Structure genes such as PAL, CHS, F3H, DFR and ANS, and regulator genes fragments such as bHLH, WD40, MYB and bZIP were screened from subtraction libraries involved in anthocyanidin biosynthesis. 从消减文库中筛选出1424个特异基因片段,其中包括花色素生物合成途径所需酶的结构基因:PAL,CHS,F3H,DFR,ANS,同时也筛选到花色素生物合成相关的调控因子的编码序列,如bHLH,WD40,MYB和bZIP。
- Objective:To identify the differentially expression of the gene perforin from the subtracted libraries between asymptomatic HBV carriers(ASCs) and the normal control subjects. 目的:验证无症状乙型肝炎病毒携带者(A SC s)与正常对照组消减文库中的差异基因穿孔素(perforin)在相应人群中的差异表达。
- Results from Blast analysis revealed 14,20,23 and 19 homolog genes in 4-w forward,4-w reverse,8-w forward and 8-w reverse subtractive libraries,respectively. 同源性分析结果提示4周正向文库得到14个同源基因，4周反向文库得到20个同源基因，8周正向文库得到23个同源基因，8周反向文库得到19个同源基因。
- Two from five is a simple subtraction. 五减二是简单的减法。
- If you subtract 2 from 8, you get 6. 8减去2是6。
- Subtract 3 from 7 and you have 4. 七减三得四。
- subtraction libraries 消减文库
- Then the subtracted library was screened by PCR and the differential fragments were sequenced and analyzed with BLAST. 对差减文库进行PCR筛选，对得到的差异片段进行测序及BLAST分析；
- Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA. 目的：消减文库构建过程中，用PCR技术快速筛选重组阳性克隆。
- The little girl has begun to learn to add and subtract. 这小女孩已在学做加减法了。
- Recombinant clone can be analyzed directly with the use of PCR.It is very efficient,especially in establishment of subtractive library of cDNA. 筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
- We do addition and subtraction in arithmetic. 在算术里，我们作加减运算。
- If you subtract 3 from 5, you get 2. 五减去三就得二。
- People who work in libraries spend a lot of time classifying books. 图书馆工作人员要花很多时间把图书分类。
- The methods in cDNA subtractive library construcdon have been reported a lot, and also developed two kinds of directions , btt no one is perfect still now. 近年来报道了多种构建方法，并形成了两个发展趋势，但目前还无一种方法被普遍认可。
- When we do subtraction,we take things away. 当我们做减法的时候，我们取走事物。
- The libraries in large cities are well supplied with books on most subjects. 大城市的图书馆藏书丰富，备有大部份类目的书籍。
- If you subtract two from five you get three. 五减去二剩下三。
- A compiled list of the contents of two or more tape libraries. 包括两个或多个磁带程序库的一种汇编目录
- The children are learning to add and subtract. 孩子们学做加减法。
