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- Then the subtracted library was screened by PCR and the differential fragments were sequenced and analyzed with BLAST. 对差减文库进行PCR筛选，对得到的差异片段进行测序及BLAST分析；
- Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA. 目的：消减文库构建过程中，用PCR技术快速筛选重组阳性克隆。
- Structure genes such as PAL, CHS, F3H, DFR and ANS, and regulator genes fragments such as bHLH, WD40, MYB and bZIP were screened from subtraction libraries involved in anthocyanidin biosynthesis. 从消减文库中筛选出1424个特异基因片段,其中包括花色素生物合成途径所需酶的结构基因:PAL,CHS,F3H,DFR,ANS,同时也筛选到花色素生物合成相关的调控因子的编码序列,如bHLH,WD40,MYB和bZIP。
- Recombinant clone can be analyzed directly with the use of PCR.It is very efficient,especially in establishment of subtractive library of cDNA. 筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
- The methods in cDNA subtractive library construcdon have been reported a lot, and also developed two kinds of directions , btt no one is perfect still now. 近年来报道了多种构建方法，并形成了两个发展趋势，但目前还无一种方法被普遍认可。
- Two from five is a simple subtraction. 五减二是简单的减法。
- If you subtract 2 from 8, you get 6. 8减去2是6。
- The college library is a favorite haunt. 大学图书馆是人们喜欢的地方。
- He is a vast library in epitome. 他脑中有万卷藏书。
- Subtract 3 from 7 and you have 4. 七减三得四。
- He gave away his books to a circulating library. 他把自己的书籍损赠给一家循环图书馆。
- The library boasts first edition of Shakespeare. 这个图书馆以藏有初版的莎士比亚集而自豪。
- I have the book on loan from the library. 我从图书馆借来这本书。
- I passed the store on my way to the library. 我在去图书馆的路上经过了那家商店。
- His hunger for knowledge drove him to the library. 他对求知的强烈渴望驱使他上图书馆。
- The library was enriched with new books. 购买新书使图书馆得到了充实。
- Students must have access to a good library. 学生要有使用好图书馆的便利条件。
- The little girl has begun to learn to add and subtract. 这小女孩已在学做加减法了。
- You can look for the book in the library catalog. 你可以在图书馆的图书目录上查找这本书。
- Keywords wheat powdery mildew;Benzothiadiazole （BTH）;systemic acquired resistance;pathogenesis-related proteins;suppression subtraction library; 小麦白粉病;BTH;系统获得性抗性;病程相关蛋白;抑制性消减杂交文库;
