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- Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA. 目的：消减文库构建过程中，用PCR技术快速筛选重组阳性克隆。
- Recombinant clone can be analyzed directly with the use of PCR.It is very efficient,especially in establishment of subtractive library of cDNA. 筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
- The methods in cDNA subtractive library construcdon have been reported a lot, and also developed two kinds of directions , btt no one is perfect still now. 近年来报道了多种构建方法，并形成了两个发展趋势，但目前还无一种方法被普遍认可。
- Results from Blast analysis revealed 14,20,23 and 19 homolog genes in 4-w forward,4-w reverse,8-w forward and 8-w reverse subtractive libraries,respectively. 同源性分析结果提示4周正向文库得到14个同源基因，4周反向文库得到20个同源基因，8周正向文库得到23个同源基因，8周反向文库得到19个同源基因。
- Then the subtracted library was screened by PCR and the differential fragments were sequenced and analyzed with BLAST. 对差减文库进行PCR筛选，对得到的差异片段进行测序及BLAST分析；
- Keywords aloe;gene;salt-stress;SSH;subtractive library;NADP-ME; 芦荟;基因;盐胁迫;抑制消减杂交;消减文库;NADP-苹果酸酶;
- Construction of Fetal Mesenchymal Stem Cell cDNA Subtractive Library 胎儿间充质干细胞cDNA扣除文库的构建
- The college library is a favorite haunt. 大学图书馆是人们喜欢的地方。
- If you subtract 2 from 8, you get 6. 8减去2是6。
- He is a vast library in epitome. 他脑中有万卷藏书。
- Subtract 3 from 7 and you have 4. 七减三得四。
- He gave away his books to a circulating library. 他把自己的书籍损赠给一家循环图书馆。
- The library boasts first edition of Shakespeare. 这个图书馆以藏有初版的莎士比亚集而自豪。
- I have the book on loan from the library. 我从图书馆借来这本书。
- I passed the store on my way to the library. 我在去图书馆的路上经过了那家商店。
- His hunger for knowledge drove him to the library. 他对求知的强烈渴望驱使他上图书馆。
- The library was enriched with new books. 购买新书使图书馆得到了充实。
- Students must have access to a good library. 学生要有使用好图书馆的便利条件。
- You can look for the book in the library catalog. 你可以在图书馆的图书目录上查找这本书。
- The little girl has begun to learn to add and subtract. 这小女孩已在学做加减法了。
