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- The transfection reagent lipofectin and the mismatch control oligonucleotide did not significantly affect the current. 而核酸转染剂阳离子脂质体和非配对性的寡核苷酸对电流没有显著性影响。
- With FuGENE6 transfection reagent, AIRE gene was stably expressed in HeLa cells that would be helpful for further study on AIRE gene. 在 Fu GENE6转染试剂的介导下 ,AIRE基因在 He La细胞内获得稳定表达
- Recombinant retrovirus was produced by transient transfection of the Phoenix-ecotropic packaging cell line with pMX-3FLAG-Id3-EGFP vector using FuGene-6 transfection reagent. 通过脂质体转染技术将重组逆转录病毒载体(pMX-3FLAG-Id3-EGFP)转染Phoenix-ecotropic包装细胞系;
- Methods: Eukaryotic expression vector plasmid containing AIRE cDNA was transfected into HeLa cells with FuGENE6 Transfection Reagent. 方法 :用 Fu GENE6转染试剂将含 AIRE c DNA的真核表达载体转染 He La细胞 ,经 G41 8筛选阳性克隆。
- Objectives: To explore the functional property of fusion protein encoded by pGJA-P/VAX1. Methods: COS-7 cells were transfected with pGJA-P/VAX1 or pVAX1 using sofastTM transfect reagent. 目的:体外检测靶向融合防龋DNA疫苗pGJA-P/VAX1编码蛋白的生物学活性,研究蛋白是否具有靶向于抗原递呈细胞如树突状细胞的能力。 方法:pGJA-P/VAX1和pVAX1分别转染真核细胞COS-7,收集并浓缩细胞培养上清。
- Human brest cancer cell MCF-7 was transfected with pcDNA3.1(-)/VEGF-C by using lipofectamine transfection reagent and screened with antibiotic G418. The expression of VEGF-C protein in CHO cell and supernatant was identified with Western-blotting. 最后用Western -blotting法检测细胞培养上清以及裂解细胞中的VEGF -C蛋白。
- transfection reagent 转染试剂
- The reagent possesses specificity for water. 这种试剂对水有特殊的作用。
- L-Methyl glyoxylate is an important chiral reagent. 二羟乙酸L-薄荷酯是一种重要的手性试剂。
- This reagent attacks feldspars and dark minerals. 这种反应物质侵蚀长石和音色矿物。
- Luoyang Tiancheng Chemical Reagent Co., Ltd. 洛阳天骋化学试剂有限公司。
- Tianjin Damao Chemical Reagent Co., Ltd. 天津大茂化学试剂公司。
- Effects of transfection reagents and Helicobacter pylori on morphology of AGS cells 转染试剂及幽门螺杆菌对AGS细胞形态的影响
- BEIJING HENGJU OILFIELD CHEMICAL REAGENT CO., LTD. 北京市恒聚油田化学剂有限公司。
- They often use oriole reagent in their experiments. 他们在实验过程中,要经常用到吡咯试剂。
- After ATF gene transfection, the ATF mRNA was obviously expressed. 结果表明,转染ATF基因后,肿瘤细胞呈现ATF基因的明显表达;
- Transfection, make stable C/EBP p expression cell line. 质粒转染,制备稳定表达C尼BPp的细胞系。
- Cell density was associated with PEI/DNA transfection efficiency. 细胞密度影响转染效率;
- Gene transfection is the key technique of gene therapy. 摘要基因治疗的关键技术是基因转移。
- This bifunctional reagent links protein molecules to one another. 这种双用试剂把蛋白质分子彼此连接起来。