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Increasing the Transient Expression of GUS Gene in Plant Tissue by Homologous Recombination. 利用同源重组提高外源GUS基因在植物组织中表达。
The COS cells were transinfected by pC-IL-2 using DEAE-Dextran method for transient expression of IL-2 gene. 采用DEAE-Dextran介导的方法,用IL-2真核表达载体pC-IL-2转染COS细胞进行暂态表达。
But MARs had no effect on the GUS gene expression levels in transient expression. 但在瞬时表达中 ,MARs对GUS基因的表达没有影响。
The results were that transient expression had not obtained after transformating dry seeds implanted. 结果表明：直接注入干种子后进行转化，未得到瞬间表达的结果。
Transient expression of the RZF71-GFP protein in onion epidermal cell showed that RZF71 was localized in cell nuclei. 农杆菌介导的洋葱表皮细胞GFP瞬时表达实验表明：RZF71定位于细胞核内。
The transient expression vector--pUC-pBin was built, the gene VirD_1, VirD_2, VirE_2 and GFP was joined in it respectively. 构建了瞬时表达载体pUC-pBin，并将目的基因VirD_1，VirD_2，VirE_2和GFP分别连在瞬时表达载体上。
Transient expression vectors were constructed by fusing the promoter fragment with gus reporter gene and nopaline terminator in different orientation. 将启动子片段分别以不同方向与gus报告基因和nos终止子融合 ,构建了瞬时表达载体。
By detecting the transient expression of GUS gene, the factors affecting the early phase of Agrobacterium-mediated transformation were investigated. 通过测定GUS基因的瞬时表达率，对影响根癌农杆菌介导的香蕉基因转化早期主要因素进行了研究。
By fusing this two fragment with gus reporter gene and nopaline terminator, we get two transient expression vectors which named pCAMBIA-ACTP and pBI-ACTPS. 将该启动子两个片段分别与gus报告基因和nos终止子融合，构建成瞬时植物表达载体pCAMBIA-ACTP和pBI-ACTPS。
But if 2-day-seedling was implanted, and then dipped into DNA solution, the transformation effect was increased evidently. 10% transient expression was obtainted. 而注入玻璃化保护后的2天龄幼苗，再进行转化，转化效果明显提高，得到了大约10%25的瞬间表达。
As SV40 plasmids replicate out of control in COS7 cells and be lethal to such transfected cells, SV40 vector/COS7 cell system has only been using as transient expression system for a long time. 基于猴病毒40(SV40)载体在COS7细胞内的复制没有节制并对细胞具有致死性,长期以来SV40载体-COS7系统只被用作瞬时表达。
Objective To establish a transient expression system of mouse DVL-1cDNA recombinant plasmid in cultured wild-type mouse Neuroblastoma 2a (N2a), for the further use in studying the role of Wnt signaling in Alzheimer disease pathogenesis. 目的将鼠dishevelled 1(DVL 1)cDNA重组质粒转染到培养的鼠成神经瘤细胞N2a ,建立瞬时表达系统 ,为研究Wnt信号途径在阿尔茨海默病 (AD)发病中的作用提供实验基础。
Exect gene setuence hVEGF165 was obtained from the fetal human myocardium tissue by RT PCR; pcDNA3.1/VEGF165 was constructed ; and transient expression was going after transfecting COS 7 cell. RT PCR方法从胎儿心肌组织获得正确的hVEGF165基因序列,成功构建pcDNA3·1 /hVEGF165且实现转染COS 7细胞的瞬时表达。
Methods: The transient expression system of COS7 was constructed by using pcDNA3.1-sEGFR501 expression vector and the process of Calcium-phosphate co-precipitation transfection was optimized with Enhanced Green Fluorescent Protein (EGFP) as a reporter. 方法：以增强型绿色荧光蛋白(EGFP)基因为报告基因，优化磷酸钙共沉淀转染COS7的实验参数；以pcDNA3.;1-sEGFR501和COS7组建瞬时表达系统。
Two recombinant human granulocyte colonystimulating factor expression plasmids pED-GCSF and pEF-GCSF were constructed. Each plasmid was transfected into the COS7 cells for transient expression,and into CHO-dhfr - cells for stable expression. 利用PCR反应、DNA测序、基因重组等技术，构建了两个表达人粒细胞集落刺激因子cDNA的重组质粒pED－GCSF和pEF－GCSF，两质粒分别转染COS7细胞作瞬时表达，转染CHO－dhfr－细胞作稳定表达。
Objective To construct recombinant human bone morphogenetic protein 2 (rhBMP2) eukaryotic expressing vector, and to transfect human fibroblasts and other two cell types, and to determine the transient expression of BMP2 in these cells. 目的构建人骨形成蛋白2(BMP2)的真核表达载体以探讨成纤维细胞表达BMP2的情况。
The results suggested that the PEBV transient expression system has been established successfully and the GFP gene has been spread systemically and expressed at high level in Bean Pod. 结果表明，本实验成功地建立并优化了以PEBV为载体的外源蛋白瞬时表达体系，报告基因gfp实现了在豆角中的系统扩散和大量表达。
An expression of pain flitted across her face. 她脸上闪过一种痛苦的表情。
Her feeling of depression was transient. 她那抑郁的心情一会儿就过去了。
He always shows a doleful expression. 他总是露出忧郁的表情。