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- The segments amplified with 3 cpDNA and 3 mtDNA universal primers were digested by 15 restriction endonucleases. 3个叶绿体和3个线粒体通用引物扩增出的片段,用15种限制性内切酶对其进行酶切。
- However,false-positives can not be avoided when bacterial ribosomal RNA gene was amplified with universal primers. 但是,在使用通用引物扩增细菌基因时,假阳性很难避免。
- The detection method for various enteroviruses in water sample by RTPCR with universal primers was established. 建立了一种利用通用引物RT-PCR技术检测水中肠道病毒的方法。
- Amplification and Orientation of Dengue 2 Virus Partial E Gene Insert in pUC18 by Universal Primer Directed PCR. 通用引物PCR分离登革病毒2型cDNA及其插入方向鉴定
- The universal primer(CHS1 1S,CHS1 1R)is regarded as a specificity primer. The result showed that the sensitivity of universal primer PCR is 100 fg. 皮肤病原真菌通用引物(CHS1 1S,CHS1 1R)具有较强的特异性,敏感度为100 fg;
- Using the adapter-ligated fragments as templates, a 7-plex allele-specific am-plification was performed by 7 allele-specific primers and a universal primer in one tube. 以连接产物为模板, 在两管中分别用7条等位基因特异性引物和一条公用引物进行7重等位基因特异性扩增;
- Sensitivity determination of universal primer PCR: Seven samples ranging from 100 ng to 100 fg shown positive,except the 10 fg sample yielding negative band. 皮肤病原真菌通用引物PCR敏感性测定,模板DNA 100 ng~100 fg的7个系列浓度均扩增出了阳性条带,10 fg为阴性。
- Methods To amplify the class1,2 and 3 integrase genes in 184 gram negative bacilli from medistream urine by polymerase chain reaction with universal primer of 3 genes. 方法用1、2、3类三种整合酶基因通用引物扩增184株中段尿中分离革兰阴性杆菌的相应基因;
- The partial bla gene of ESBL producing isolates and their transcojugants were detected by PCR using universal primers for TEM, SHV, CTX-M-1group, Toho-1group, CTX-M-13group respectively. TEM、SHV、CTX M 1组、CTX M 13组、Toho 1组通用引物检测产ESBL菌株及其转移接合子 ;
- Method Acoording to the analysis of the conservative and variable regions in bacterial 16S rRNA genes , we designed universal primers for all bacteria and specific primers for most gram-positive and gram-negtive bacteria . 方法 通过对多数病原菌16S rRNA基因保守区和变异区的序列分析 ,设计通用引物和革兰阴性菌及革兰阳性菌的特异性引物分别作为外侧和内侧引物,建立了对不同细菌的16S rRNA基因进行多重半套式PCR扩增方法;
- Methods A set of universal primers was designed based on the conservative regions in bacterial 16S ribosomal RNA genes. 7 control bacterial strains,human DNA,HBV-DNA and 37 samples of ascites of patients with cirrhosis were amplified with PCR. 方法在细菌16S rRNA基因保守区设计一对通用引物,对7种对照菌株、人基因组DNA、HBV-DNA、37份肝硬化患者腹水进行PCR扩增。
- The genomic DNA was isolated and purified with the improved CTAB methods. The sequences of 16S rRNA gene were amplified by TD-PCR with the bacterium universal primers and the purified PCR products were directly sequenced. 菌株经纯化培养;以改良CTAB法提取总DNA;采用细菌16S rRNA通用引物、TD-PCR方法(touchdown-PCR)进行16S rRNA基因序列扩增;PCR产物纯化后直接进行序列测定;序列经人工校对后用Clustal X进行比对分析;最后用MEGA3.;1软件构建系统发育树。
- Children learn knowledge from primers. 孩子们从启蒙书上学习知识。
- I borrowed from the library a primer of phonetics. 我从图书馆借了一本语音学入门书。
- Keywords 23S rDNA;gene chip;universal primers;bacteria infection; 基因芯片;共同引物;细菌感染;
- Overpopulation is a universal problem. 人口过多是全球性的问题。
- Music has been called the universal language. 人们称音乐为世界的语言。
- His courage compels universal admiration. 他的勇气不禁令人肃然起敬。
- Universal Primer PCR for Detection of Clinic Bacteria 通用引物PCR检测临床常见致病菌的实验研究
- Beijing Technology and Business Univ. 北京科技大学外语系。