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The overall virulence gene prevalence was 62.7% (42/67); and the main virulence gene type was slt_1+ slt_2+eaeA+hly. 62.;7%25的株菌(42/67)携带有毒力基因;毒力图谱类型主要为slt1+slt2+eaeA+hly。
By the colony hybridization with the virulence gene brobes of ctx, zot, ace, and RS1, all of CVC were positive. 用ctx、zot、ace、RS1为探针，对471株O1群霍乱弧菌进行毒力基因检测；结果：CVC菌株全部阳性；
Also covered are methods for studying gene expression and detecting virulence genes. 此外包括是用于研究基因表示和检测有毒基因方法。
These results indicate that the major virulence genotype of Anhui isolation strains is the high virulence phenotype of alt~+aha1~+aerA~+ and the virulence gene alt generally exists in different Aeromonas phenospecies. 表明气单胞菌安徽分离株的主要毒力基因型是alt+aha1+aerA+的高毒力表型,alt毒力基因普遍存在于不同表型种气单胞菌中。
Adhesion gene (aha1), aerolysin gene (aerA) and cytotonic enterotoxin gene (alt) were considered as major virulence genes of Aeromonas. 粘附素(aha1)、气溶素(aerA)和细胞兴奋性肠毒素(alt)是气单胞菌的主要毒力因子。
Methods By RT-PCR,specific amplification products of P2 gene from H2 final product and its parent seed were obtained,then sequenced and blasted to analyze its virulence gene VP1/2A and 2C. 方法通过逆转录-聚合酶链反应,特异扩增甲肝减毒活疫苗H2株成品疫苗及其亲本毒种P2区VP1/2A、2B、2C基因序列,经测序、比对,分析检测毒力基因VP1/2A、2C的变异情况。
Some pathogenicity was steadily inherited, which suggested that the virulence genes controlling the pathogenicity appeared to be homozygous nuclear gene. 控制大豆疫霉菌的致病基因分布在不同位点，有的遗传稳定，由纯合的核基因控制；
In 20 grade 1 ulcers (92%), no virulence genes were identified whereas these genes were present in all but one grade 2-4 ulcers. 除了1名病人外，致病基因在所有2-4级溃疡病人中都被检测到，而在1级溃疡病人中，20名(92%25)未检测出致病基因。
On the contrary, two grade 1 ulcers healed: the genotype profiles were different from those at inclusion but without appearance of virulence genes. 相反，两名1级病人治愈后，基因型却不同于那些包含但未表现的致病基因。
Pseudomonas is also known to switch on its virulence genes in response to signals from quorum sensing. 据了解假单细胞菌也会打开它的有毒基因以响应来自群体的信号。
According to the four types of virulence genes: stx1, stx2, eaeA and hlyA, four pairs of specific primers have been designed. [方法]针对产志贺毒素大肠杆菌(STEC)的stx1、stx2、eaeA、hlyA4种毒力基因,设计了4对特异性引物。
Methods Virulence genes, including stx1, stx2, EPEC and EHEC general eaeA fragment (eaeA-gen), were amplified by multiplex PCR in E. coli strains. 方法应用多重PCR同时检测大肠埃希菌菌株的stx1、stx2、EPEC和EHEC共同的eaeA(eaeA -gen)等靶基因 ;
There are also unknown virulence factors being identified with exception of known virulence genes identified in all these screens. 这些筛选中除了找到已知的毒力基因外,还都鉴定到了未知的毒力因子。
Using oligonucleotide arrays, S. aureus resistance and virulence genes were compared between grade 1 and grade 2-4 ulcers. 应用寡核苷酸芯片后，比较1级和2-4级溃疡病人中，金黄色葡萄球菌的耐药性和致病基因。
During follow-up, the two grade 1 ulcers which infected with strains carrying virulence genes rapidly deteriored: the array technology showed unchanged genotype profiles. 在随访中，感染携带致病基因的两名1级病人病程急剧恶化：芯片方法显示基因型未发生改变。
Of forty eight isolates carrying virulence genes, 38( 79.2%) had SLT 2, eaeA and hly genes, taking the dominate virulence gene pattern, 8( 16.6%) had all of the four virulence genes 2( 4.2%)had both SLT 2 and hly genes respectively. 菌株毒力基因图谱以SLT2 +eaeA +hly为主 ;占 79.;2%25;其次为SLT2 +SLT1+eaeA +hly和SLT2 +hly;分别占 16
Keywords S.typhi;R plasmid;Virulence gene; 伤寒杆菌;耐药质粒;毒力基因;
The assay was designed to amplify the 1109, 302 and 228 bp regions of corresponding virulence genes eaeA, hlyAB, slt1 and /or slt2, by using specific primers for each one reaction. 将样品增菌后 ,采用快速裂解吸附法制备模板 ,用多重PCR同时检测EHEC的三种毒力基因eaeA、hlyAB、slt1 2 ,相应扩增片段依次为 110 9、30 2、2 2 8bp。检测了 6 0株大肠杆菌和其它菌种。
Method The virulence genes of Shiga like toxin(SLT 1 and SLT 2), intimin(eaeA) and hemolysin(hlyA) were chosen as the target genes and amplified in multiplex PCR assays. Results Of the eighty five E. 方法应用肠出血性大肠杆菌 (EHEC)的多重引物聚合酶链反应 (PCR)方法 ,以志贺样毒素 (SLT2 和SLT1)基因、“粘附抹平”因子eaeA基因和溶血素 (hly)基因为靶基因进行检测。
AiiA protein, blocking the bacterial quorum sensing by hydrolyzing AHL-lactone, can greatly attenuate the disease caused by many bacterial pathogens in which quorum sensing regulate the expression of virulence genes. AiiA蛋白通过破坏病原菌的信号分子来阻断病原菌的群体感应调节系统;达到抗病的目的;是一种新型的抗病蛋白.