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- The anion currents elicited by swelling rat ventricular myocytes in 1T and 0.6T solution, namely ICl,swell were studied using patch clamp techniques with whole - cell voltage clamp configuration. 采用全细胞电压钳方式记录大鼠心室肌细胞在1T及0.;6T溶液中ICl;swell的变化。
- In this work, by reconstituting the channel protein into lipid bilayer, Ca2+ channels in human spermatozoa were investigated with voltage clamp technique. 本工作将通道蛋白重组于脂双层,在电压箝位下记录了人精子膜的Ca2+通道电流。
- whole-cell voltage clamp technique 全细胞式膜片钳技术
- voltage clamp technique 电压钳制术
- Methods Whole cell patch clamp techniques were used to investigate the effects of 2-APB on Isoc in human hepatocytes. 方法应用全细胞膜片钳记录技术测定人肝细胞Isoc,并观察2-APB对该电流的影响。
- Transistors Q1 and Q2 form a voltage clamp network.This clamp performs a current-limiting function on the overload current to U1 at 10 mA during ohms and temperature overloads. Q1和Q2实现电压钳断网络,这个钳断会在欧姆或是温度过载时使得进入U1的电流在10毫安。
- Methods Whole cell patch clamp techniques were employed to investigate the effect of aminophylline on L-type calcium cur-rent of diaphragmatic muscles in rats. 方法:采用全细胞膜片钳(whole cell patchclamp)技术;当维持电位为-80mv;刺激频率为0.;5Hz;钳制时间为300ms;步进电压为10mv;去极到60mv;观察氨茶碱对膈肌细胞L型钙通道(L-ICa)的影响。
- ABSTRACT:Objective To investigate the method of stably isolating pachyntic Spraque Dawley rat calcium tolerant ventricular myocytes for patch clamp techniques. 随着心肌细胞电生理和分子生物学研究的兴起,成熟单个心肌细胞越来越多的应用于心血管疾病病理生理的研究。
- Methods Front wall midmyocardial of left ventricular were isolated from New Zealand white rabbits . ICa-L in 10-6,10-5mol/L ibutilide was recorded using whole cell patch clamp techniques. 方法用全细胞膜片钳技术记录10-6,10-5mol/L伊布利特细胞外液对兔正常左室中层心肌细胞L型钙通道电流(ICa-L)活性的影响。
- Whole cell voltage clamp recording 全细胞电压钳记录
- METHODS: The acetyl-choline (ACh)-induced current (IACh) was measured on PC12 cells by whole-cell clamp technique. 方法:采用全细胞膜片箝技术记录PC12细胞乙酰胆碱诱发电流(I_(ACh))。
- For the past year, I have been working with a subset of neuronal glutamate receptors via voltage clamping on Xenopus oocytes. 过去的一年里,我一直在研究在蛙卵上神经元谷氨酸受体的一个子集通过电压箝位的现象。
- Abstract: The study of lens cells by patch clamp technique began in th e early 90"s. 文摘:应用膜片钳技术对晶体细胞的研究始于90年代初。
- Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent. 方法 :采用全细胞膜片钳技术 ,记录钠通道电流。
- Fasting plasma cortisol, insulin, glucose and insulin sensitivity (with euglycemic clamp technique were measured. 测定空腹皮质醇、葡萄糖、胰岛素水平及胰岛素敏感性(葡萄糖钳夹技术评价)。
- Method Ion currents of rabbit ventricular myocyte were recorded using the whole cell patch clamp technique. 方法应用全细胞膜片钳技术记录兔心室肌单个细胞的电流。
- She initiated euglycemic clamp technique in China which is considered as the “Golden standard” to test insulin sensitivity and insulin secretion. 她在我国建立了葡萄糖钳夹技术,该技术被认为是测量胰岛素敏感性和胰岛素分泌的“金标准”。
- Input Filter and Voltage Clamp Circuit Design of Matrix Converter 矩阵变换器输入滤波器和箝位电路设计
- Methods Whole cell patch clamp technique was used to test the effects of Pho and Fen on TTX-S and TTX-R sodium currents in DRG neurons. 方法应用膜片钳技术研究氰戊菊酯和辛硫磷对DRG河豚毒素敏感(TTX-S)和不敏感(TTX-R)型钠通道的作用。
- Action of ion channel on mem brane is the key events of messenger transduction for excitable cells,which can be detected by the patch clamp technique. 离子通道的活动是可兴奋细胞传递信息的基础,膜片钳技术的发明导致了生命科学的一场革命。