was carried out. The fit inducing culture media of Viola diffusa Ging. calli which were MS+2,4 D 1 5+ZT 1 0 was sieved out by the experiment L 9(3 4).

• 通过 L9( 34)正交试验筛选出诱导蔓茎堇菜愈伤组织的适合培养基为 MS+2 ；4 -D1 .；5+ZT1