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- . 2.重组表达质粒转染后的AAV-293细胞可检测到报告基因绿色荧光的表达,根据表达荧光的细胞数得到重组病毒的感染性滴度为106 particles/ml。GFP expression in co-transfected AAV-293 cells was observed and the titer of rAAV is 106particles/ml measured by that cells expressing green fluorescence were counted and compared with the total number of cells.
- 建立了用超临界制粒的三种方法:快速膨胀超临界溶液(Rapid Expansive of Supercritical Solutions,即RESS)、超临界反萃剂(Supercritical Antisolvent,即SAS)和气体饱和溶液(Particles from Gas-Saturated Solution,即PGSS)方法。Particles for Supercritical Fluid (PSF) includes Rapid Expansive of Supercritical Solutions (RESS), Supercritical Antisolvent (SAS) and Particles for Gas-Saturated Solutions (PGSS) or CO2-Assisted Nebulization Bubble Drying(CAN-BD). PSF overcomes the disadvantages of traditional produced by this approach can be within micro/nanometer. And size with uniform distrubution.
