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- 方法:改良CTAB法。Methods: The improved CTAB DNA extraction method.
- 采用改良CTAB法提取河南 17个桂花品种的基因组DNA ,利用RAPD技术对其进行鉴定和分类研究。Applying an improved CTAB method to extract DNA from leaves of Osmanthus fragrans, studied the classification and identification of 17 Osmanthus fragrans cultivars in Henan province by the technique of random amplified polymorphic DNA(RAPD).
- 改良CTAB法提取DNA质量好于改良SDS法,改良方法尤其改良CTAB法是一种适合小麦基因组DNA提取的方法。The improved CTAB extraction method which was designed by our research group is suitable to extract the high quality DNA from wheat. Facile operation can save time and be economical.
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- 用改良CTAB法提取的总DNA的OD260/OD280在1.6-2.0之间,电泳条带清晰,其含量和纯度完全满足RAPD分析的要求。The OD260/OD280 of total DNA was between 1.6 to 2.0 with the improved method of CTAB,the banding patterns was clear,and the content and pureness of the total DNA can meet the reaction for RAPD.
- 改良improve
- 用法usage
- 说法statement
- 改进的CTAB法DNA得率高于改进的SDS法;DNA yield rate of modified CTAB method was higher than modified SDS method.
- 改良的improved
- 输入法input method
- 改良CTABCRAB
- 除法division
- 分析法analytical method
- PCR方法检测转基因食品时,应用改良CTAB法制备DNA模板。DNA extract by using modified CTAB methods was suitable for PCR-based detection of genetically modified organisms in foods and animals feed.
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- 宾夕法尼亚Pennsylvania
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