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- 地高辛标记引物酶显色法检测HBV基因多态性及应用Application of enzyme coloration with digoxin-labeled primers for detection of HBV gene polymorphism
- 方法 应用聚合酶链反应（PCR）方法，采用荧光标记引物和377型DNA序列自动分析仪，分析21例 GBM 9号染色体上 20个微卫星多态性标记的LOH。Methods 20 loci on chromosome 9 were examined to detect loss of heterozygosity(LOH) in 21 cases of glioblastoma by PCR based microsatellite polymorphism analy-ses, in which fluorescence-labeled primers and Perkin Elmer 377 DNA Sequencer were applied.
- 荧光标记引物复合扩增短串联重复序列技术用于异基因造血干细胞移植植活测定A study on technique of multiplex polymerase chain reaction with fluorescence labeling primers for short tandem repeats to document engraftment following allogeneic stem cell transplantation and its application
- 任意引物PCRarbitrarily primed PCR
- 标记mark
- 二引物PCRTP-PCR
- 双引物PCRDouble - primer PCR
- 方法 应用四色荧光标记引物复合扩增技术 ,对鄂伦春族 10 1名无关个体的血样 15个STR基因座进行分型研究 ,计算各基因座的相关群体遗传学参数。Method DNA samples from 101 unrelated individuals in Chinese Olunchun population were screened by Power Plex 16 System and ABI 3100 Genetic Analyzer.
- 引物primer
- 随机引物PCRarbitrarily primed PCR(APPCR)
- 组合引物PCR法grouping primers PCR
- 半随机引物PCRSemi-random primer PCR
- 方法应用聚合酶链反应(PCR)方法,通过荧光标记引物和377型DNA序列自动分析仪,对20例原发性胶质母细胞瘤患者7号染色体上的22个微卫星多态性标记进行杂合性丢失(lossofheterozygosity,LOH)分析。Methods All of the 22 microsatellite polymorphism markers on chromosome 7 in 20 cases with GBM were detected by polymerase chain reaction (PCR) technique with fluorescence-labeled primers and Perkin Elmer 377 DNA sequencer and the loss of heterozygosity (LOH) in them was analyzed.
- 引物原位标记primed in situlabelling; RINS
- 型特异性引物PCRType-specific primer PCR
- 荧光标记引物fluorescent dye primer; fluorescently-labeled primer
- 随机引物法标记Random Prime Labeling of DNA
- RNA任意引物PCRRNA arbitrarily primed PCR
- 随机引物标记法random primering
- 因而，设计PCR引物时宜选择Takahashi等分离株为标准。In China these viruses share high homology with Takahaski’s strains which might be good standards for primer designation.
