A CTV strain,T514,is used for the preparation of inject antigen. Hybrids are cloned by limiting dilution and screened for reactivity to sap from CTV infected and healthy citron.

• 免疫小鼠的CTV株系为T514；杂交瘤细胞以有限稀释法获得纯克隆；并以健康枸橼和CTV不同株系感染枸橼的组织浸出液筛选各克隆；并确认各克隆与CTV各株系的免疫反应性.