After incubation with Cpd 861 for 48 hours, HSC was harvested to determine collagen type I,III,IV mRNA by dot blot hybridization and cell culture medium was used to detect collagen secretion by ELISA method.

• 传一代细胞以10mg/ml复方861作用48小时后 ,以ELISA法、斑点杂交法检测胶原蛋白分泌及相应mRNA水平的变化。