Antigen was appraised by peptide mass fingerprint (PMF) matched with Mascot human protein database. PMF was obtained by immunoprecipitation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).

• 用免疫沉淀和基质辅助激光解吸电离飞行时间质谱(matrix-assistedlaserdesorption/ionizationtime-of-flightmassspectrometry,MALDI-TOF-MS)得到相应的肽质量指纹谱(peptidemassfingerprint,PMF),再通过Mascot在人类蛋白质数据库中分析比对鉴定mAb对应的抗原。