Apoptotic cells were detected by TUNEL assay in 1h,1d,3d,7d,10d,14d,28d after the establishment of HRD. The expressions of p53 gene protein and mRNA in the models of HRD and RD were evaluated with immunohistochemistry and in situ hybridization.

• 分别在手术后1h和1、3、7、10、14、28d对视网膜进行细胞凋亡检测实验,并采用免疫组化染色和原位杂交检测方法观察p53在视网膜中的表达情况。