CMS sorghum A 1T x623A/B and A 1 V 4A/B as plant material were used in present experiment for optimizing the factors influencing RAPD reaction effect.

• 以高粱细胞质雄性不育系和保持系 A1Tx6 2 3 A/B,A2 V4 A/B为材料 ,考察了 Mg2 + ,Taq酶 ,d NTP,引物 ,模板等的用量、循环参数及不同的 PCR仪对随机扩增多态性 DNA(RAPD)反应的影响。