Chimeras were made by injecting the ES cells into C57BL/6J blastocysts,and the germline compositions of the chimeras were detected by mating them with albino ICR mice.

• 通过囊胚显微注射法，将ES细胞注入C57BL/6J胚胎中，制作了嵌合体，并通过对嵌合体后代毛色的观察，判断了嵌合体生殖细胞的组成。