Densitometric analysis of the gel indicated that this protein represented 10. 5% of total cellular proteins. The fusion protein was cleaved by CNBr, and the yield of biologically active HV3 was about 20 ATU/ml culture.

• 该质粒在大肠杆菌 JM105宿主细胞中表达后，融合蛋白（15.；6 kD）占细菌总蛋白 10 5%25，该融合蛋白经 CNBr切割释放出活性水蛭素分子，抗凝血活力达约 20 ATU/ml培养液，为以后进一步研究打下了基础。