In conclusion, co-cultured with fresh primary oviductal epithelial cell along with addition of 10%BFF in SOFaa could improve development of nuclear transferred bovine embryo in vitro.

• 表明应用新鲜原代输卵管上皮细胞进行牛核移植胚胎的共培养,并在SOFaa添加10%25BFF能够有效促进核移植胚胎的体外发育。