In order to establish a specific detecting method to AEV, monoclonal antibody against AEV was prepared with AEV purified by cesium-chloride equilibrium centrifugation propagated using SPF eggs via the yolk sac inoculation.

• 国外已有用AEV单克隆抗体(VR9-1株)建立免疫组化、间接免疫荧光和免疫过氧化物酶染色、抗生物素蛋白-生物素-过氧化物酶染色方法检测AEV抗原的成功报道,并用于AEV的定量研究,而国内相关报道较少,成功应用的更少。