In vitro screening of seed-derived and microspore-derived callus lines was carried out by the biotechnology of plant cell culture, with the raw toxin extract of piricularia oryzae as stress factor.

• 利用植物细胞培养离体筛选生物技术,以稻瘟病菌粗毒素提取液作为外源胁迫因素,对水稻种子体细胞和花粉性细胞无性系,进行愈伤组织细胞培养抗性筛选或花药培养抗性筛选。