Integration and transcription on RNA level of HTP gene and AtNHX I gene in transgenic calli were comfirmed by PCR and RT-PCR.

• PCR及RT-PCR检测结果表明:潮霉素磷酸转移酶基因及AtNHX I基因已成功地整合到苜蓿基因组中,并在RNA水平上进行了转录。