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- The content of MDA in liver homogenate was measured by TBA colorimetric assay. 用硫代巴比妥酸(TBA)显色法测定肝匀浆中MDA含量。
- The levels of ET,ALT and liver homogenate NO in HFG and HCG were higher than those in NG. 血浆ET和ALT、肝组织匀浆NO在HF组和HC组明显高于NG组。
- The metabolizing method was established based on the in vitro metabolism by Sprague-Dawley (SD) rat liver homogenate. 采用液相色谱-质谱联用方法分析了中药大黄经过SD大鼠肝匀浆体外代谢前后的指纹谱中色谱峰面积、保留值的差异。
- The ALT,AST level in serum and MDA content,SOD,GSH-PX activites in liver homogenate,Pathology of liver were observed. 结果:复方黄根可明显降低小鼠血清中ALT、AST活性,同时能减少肝匀浆MDA含量,并能使降低的肝匀浆SOD、GSH-PX活性升高。
- Methods The liver index, level of SGPT, content of MDA of liver homogenate, and degree of liver cell injury were investigated in mice. 方法分别测定肝损害组和白头翁组小鼠的肝SGPT,肝指数,肝匀浆丙二醛以及肝细胞镜检,并与对照组比较。
- The SOD activity and the concentration of MDA in rats liver homogenate were investigated to ascertain the extracting conditions. 通过考察小鼠肝匀浆中的SOD活性、MDA含量,确定最佳提取方法。
- At the time points of 0,6,12,24h, the content of serum alanine aminotransferase(ALT) , aspartate aminotransferase (AST ) and liver homogenate IL -18 were measured. 检测两组不同时间点(0、6、12、24小时)血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、肝匀浆IL-18的水平。
- Methods The hemolysis of red blood cells by itself and H2O2,MD A production in liver homogenate or induced by Fe2++VitC were measured by colo rimetric assay. 方法采用红细胞自氧化溶血、H2O2所致红细胞氧化溶血,肝匀浆MDA生成和Fe2+诱导的肝匀浆MDA生成,用比色法测定。
- The ALT and AST level in serum and MDA,SOD,GSH-PX activites in liver homogenate were assayed and dimensions of hepatic damage in the histopathology were observed. 结果:复方黄根可明显降低小鼠血清中ALT、AST活性;
- Result:Ejiaoyishoujing can obviously increase the activition of CAT、SOD、GSH-PX in blood, and decrease the level of LPO in plasma、brain and liver homogenate of aging mice. 结果:阿胶益寿颗粒可显著提高衰老小鼠血SOD、CAT及GSH-PX活性,降低血浆、肝及脑匀浆LPO水平。
- Result: Jiannao tablet can obviously decrease the level of LOP in plasma,brain and liver homogenate, and increase the activity of SOD,CAT in blood of aging model mice. 结果:健脑片能明显降低糖代谢衰老模型小鼠脑、肝匀浆及血浆中LPO水平,明显提高糖代谢衰老模型小鼠血液SOD、CAT活力。
- The laying hens were fed with diets containing selenium and iron at high level and the changes of metabolic enzyme activities in plasma and liver homogenate were studied. 用高硒高铁日粮饲喂产蛋母鸡,观察血浆及肝匀浆代谢酶活性的变化。
- Methods: The colorimetric method was used to examine the total antioxidant activity, GSH-PX and SOD activity as well as MDA level of H2O2-induced liver homogenate. 方法:采用比色法测定薰衣草提取物的总抗氧化能力、H2O2诱导的肝损伤组织中谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)的活性和丙二醛(MDA)的含量。
- Method:The model of hepatic damage was induced by BCG+LPS. The ALT and AST level in serum and MDA,SOD,GSH-PX activites in liver homogenate were assayed and dimensions of hepatic damage in the histopathology were observed. 方法:采用卡介苗(BCG)+脂多糖(LPS)造成免疫性肝损伤动物模型,观察复方黄根对小鼠血清各(ALT)、(AST)活性及肝组织匀浆超氧化酶歧化酶(SOD)、(MDA)、谷胱甘肽过氧化物酶(GSH-PX)水平的影响,并观察肝组织病理学改变。
- Plasma LPS was detected by kinetic turbidimetric limulus test at 6 h, 12 h, 24 h, 48 h, and 72 h after injury. IL-6 secretion in liver homogenate was determined by ELISA. P-STAT3 expression in BEC was analyzed by laser scan confocal microscopy. 分别于注射LPS后6、12、24、48和72h采用动态比浊法鲎试验测定血浆LPS水平,酶联免疫吸附试验(ELISA)法检测肝组织匀浆IL-6水平,激光扫描共聚焦显微镜(LSM)检测BEC内磷酸化STAT3(p-STAT3)水平,免疫组织化学法检测BEC增殖。
- Methods: The models of hepatic damage of acute were induced by CCL4, D-GalN.The ALT, AST level in serum and MDA content, SOD, GSH-PX activites in liver homogenate, Pathology of liver were observed. 方法:采用四氯化碳、D-氨基半乳糖盐酸盐诱导小鼠急性肝损伤模型,观察复方黄根对小鼠血清ALT、AST活性及肝组织匀浆SOD、MDA、GSH-PX水平的影响,并观察肝组织病理学改变。
- The poor man has a serious disease of the liver. 这个可怜的人患有严重的肝病。
- The doctor says it's just a touch of liver. 医生说肝脏稍有些毛病。
- LH: Could you focus on one given the choice? 韩:你能专心在当中一个项目吗?
- Keywords Isoliensinine;liver homogenate;lipid peroxidation; 异莲心碱;肝匀浆;脂质过氧化;