Methods APAAP assay and in situ hybridization were used for the expression of BCL 2 protein and bcl 2 mRNA in fresh bone marrow samples from 60 cases of CML. Flow cytometry was used to assess the extent of apoptosis and cell cycle percentage.

• 方法采用碱性磷酸酶抗碱性磷酸酶免疫复合物方法和地高辛标记探针原位杂交技术检测各期CML患者60例新鲜骨髓标本中BCL?2蛋白和bcl?2mRNA的表达，并对其中19例标本进行流式细胞术分析骨髓细胞凋亡率及细胞周期。